Ric3 (BC059258) Mouse Tagged ORF Clone

CAT#: MG202225

Ric3 (tGFP-tagged) - Mouse resistance to inhibitors of cholinesterase 3 homolog (C. elegans) (cDNA clone MGC:67601 IMAGE:6408852), complete

Product Images

Circular map for MG202225

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ric3
• Synonyms: E130307J04Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202225 representing BC059258
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAAATTAATCAACAGAGTTGGACCTAATGGTGAGAGCAGAGCACAGGCTGTGACTTCTGACCAAG
  AGAAACGATTACTGCATCAGCTCCGAGAAATCACCAGGGTCATGAAAGAAGGCAAGTTCATCGACACATC
  TCCAGAGAAGGAAGCTGAGGAAGCCCCATACATGGAGGACTGGGAAGGTTACCCAGAAGAGACTTATCCA
  ATATATGACCTTTCGGATGGTATCAAGCGTAGGCAAGAAACAATCCTGGTGGATTACCCTGACCTAAAAG
  AGCCCTCTGCTGAAGAAATTGCTGAACAAATGGGAGAGATAGAAGAGGAAGGATCAGAGCGTTTGAGCTG
  GGATCATTTGCCCACTGACCCTGGAGCCCAGAAAGATAATTCTGTTGCCCCGTGTGATCCCAAACCAGAA
  TCATGCTCCTGCTGTGTTCATGAAGAGGAGGATCCTGCTGTCTTGGCAGAGAATGCTGGTTTCAGTGCAG
  ATGGCTACAGTGAGCAAGAGGAAGCCACCAAGGAAAACTTGCCCCAGGACTTTACAAATGAAGGGCTGGG
  AGTCAGCACTGATAATGCACACGTGGGTGGTATGTTGAGGAAGCGCAACCCCCAGGGTTTTGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202225 representing BC059258
  Red=Cloning site Green=Tags(s)
  
  MEKLINRVGPNGESRAQAVTSDQEKRLLHQLREITRVMKEGKFIDTSPEKEAEEAPYMEDWEGYPEETYP
  IYDLSDGIKRRQETILVDYPDLKEPSAEEIAEQMGEIEEEGSERLSWDHLPTDPGAQKDNSVAPCDPKPE
  SCSCCVHEEEDPAVLAENAGFSADGYSEQEEATKENLPQDFTNEGLGVSTDNAHVGGMLRKRNPQGFE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC059258
• ORF Size: 626 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC059258, AAH59258
• RefSeq Size: 5045 bp
• RefSeq ORF: 626 bp
• Locus ID: 320360
• Cytogenetics: 7 E3
• Gene Summary: Promotes functional expression of homomeric alpha-7 and alpha-8 nicotinic acetylcholine receptors at the cell surface. May also promote functional expression of homomeric serotoninergic 5-HT3 receptors, and of heteromeric acetylcholine receptors alpha-3/beta-2, alpha-3/beta-4, alpha-4/beta-2 and alpha-4/beta-4.[UniProtKB/Swiss-Prot Function]