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Ric3 (BC059258) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202225
Ric3 (tGFP-tagged) - Mouse resistance to inhibitors of cholinesterase 3 homolog (C. elegans) (cDNA clone MGC:67601 IMAGE:6408852), complete
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Ric3 or BC059258"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ric3
Synonyms E130307J04Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202225 representing BC059258
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAAATTAATCAACAGAGTTGGACCTAATGGTGAGAGCAGAGCACAGGCTGTGACTTCTGACCAAG
AGAAACGATTACTGCATCAGCTCCGAGAAATCACCAGGGTCATGAAAGAAGGCAAGTTCATCGACACATC
TCCAGAGAAGGAAGCTGAGGAAGCCCCATACATGGAGGACTGGGAAGGTTACCCAGAAGAGACTTATCCA
ATATATGACCTTTCGGATGGTATCAAGCGTAGGCAAGAAACAATCCTGGTGGATTACCCTGACCTAAAAG
AGCCCTCTGCTGAAGAAATTGCTGAACAAATGGGAGAGATAGAAGAGGAAGGATCAGAGCGTTTGAGCTG
GGATCATTTGCCCACTGACCCTGGAGCCCAGAAAGATAATTCTGTTGCCCCGTGTGATCCCAAACCAGAA
TCATGCTCCTGCTGTGTTCATGAAGAGGAGGATCCTGCTGTCTTGGCAGAGAATGCTGGTTTCAGTGCAG
ATGGCTACAGTGAGCAAGAGGAAGCCACCAAGGAAAACTTGCCCCAGGACTTTACAAATGAAGGGCTGGG
AGTCAGCACTGATAATGCACACGTGGGTGGTATGTTGAGGAAGCGCAACCCCCAGGGTTTTGAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202225 representing BC059258
Red=Cloning site Green=Tags(s)

MEKLINRVGPNGESRAQAVTSDQEKRLLHQLREITRVMKEGKFIDTSPEKEAEEAPYMEDWEGYPEETYP
IYDLSDGIKRRQETILVDYPDLKEPSAEEIAEQMGEIEEEGSERLSWDHLPTDPGAQKDNSVAPCDPKPE
SCSCCVHEEEDPAVLAENAGFSADGYSEQEEATKENLPQDFTNEGLGVSTDNAHVGGMLRKRNPQGFE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC059258
ORF Size 624 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC059258, AAH59258
RefSeq Size 5045 bp
RefSeq ORF 626 bp
Locus ID 320360
Cytogenetics 7 E3
Summary Promotes functional expression of homomeric alpha-7 and alpha-8 nicotinic acetylcholine receptors at the cell surface. May also promote functional expression of homomeric serotoninergic 5-HT3 receptors, and of heteromeric acetylcholine receptors alpha-3/beta-2, alpha-3/beta-4, alpha-4/beta-2 and alpha-4/beta-4.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC059258" in other vectors (4)
SKU Description Size Price
MC206998 Ric3 (untagged) - Mouse resistance to inhibitors of cholinesterase 3 homolog (C. elegans) (cDNA clone MGC:67601 IMAGE:6408852), complete, (10ug) 10 ug
$330.00
MR202225 Ric3 (Myc-DDK-tagged) - Mouse resistance to inhibitors of cholinesterase 3 homolog (C. elegans) (cDNA clone MGC:67601 IMAGE:6408852), complete 10 ug
$289.00 $300.00
MR202225L3 Lenti ORF clone of Ric3 (Myc-DDK-tagged) - Mouse resistance to inhibitors of cholinesterase 3 homolog (C. elegans) (cDNA clone MGC:67601 IMAGE:6408852), complete 10 ug
$600.00
MR202225L4 Lenti ORF clone of Ric3 (mGFP-tagged) - Mouse resistance to inhibitors of cholinesterase 3 homolog (C. elegans) (cDNA clone MGC:67601 IMAGE:6408852), complete 10 ug
$600.00

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