Rreb1 (NM_001177868) Mouse Untagged Clone

SKU MC224168

Rreb1 (untagged) - Mouse ras responsive element binding protein 1 (Rreb1), transcript variant 4, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Rreb1
• Synonyms: 1110037N09Rik; AA414966; B930013M22Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224168 representing NM_001177868; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACGTCGAATTCGCCCATTGGTTTAGAAGGCTCAGACCTGTCTTCCATCAACACCATGATGTCAGCAG; TAATGAGCGTAGCGAGTGTCACAGAGAATGGTGGGAGCCCCCAGGGCATCAAGTCCCCCATGAAACCTCC; AGGACCAAATCGGATTGGCAGAAGGAACCAGGAAACGAAAGAGGAGAAGTCTTCCTACAACTGTCCCCTA; TGTGAAAAGATTTGCACCACCCAGCACCAGCTGACCATGCACATCCGTCAGCACAACACAGACACGGGAG; GAGCTGACCACGCATGCAGTATCTGTGGGAAGTCGCTGAGCTCGGCCAGCTCCCTGGATCGTCACATGCT; GGTGCACTCTGGCGAGAGGCCTTACAAGTGTACAGTGTGTGGCCAGTCTTTCACCACCAATGGGAACATG; CACAGACATATGAAGATTCATGAGAAGGATACCAACAGTACTACAGCTGCAGCCCCTCCATCCCCTCTGA; AGCGCAGGCGGTTGTCCTCCAAAAGGAAGCTGAGTCACGATGCCGAGTCAGAAGACCCAGGACCAGCTAA; AAAGATGGTAGAAGACGGGCAGTCAGGCGATTTGGACAAGATGAGCGATGAAATCTTTCACTGCCCAGTG; TGTTTCAAGGAGTTTGTTTGCAAGTATGAACTGGAGACCCACATGGAGACCCACTCAGATAACCCACTAA; GATGTGACATTTGCTGCGTCACCTTCCGCACACATCGAGGACTGCTGCGCCACAATGCACTTGTCCACAA; GCAGCTTCCCAGAGATGCCATGGGAAGACCTTTTATCCAGAACAACCCTTCGATTCCTGCTGGCTTCCAT; GATTTAGGGTTTACTGACTTCTCCTGTAGGAAGTTTCCTCGAATCTCTCAGGCCTGGTGTGAGACAAACC; TACGGCGGTGCATCAGCGAGCAGCACCGGTTTGTGTGTGACACCTGCGACAAGGCGTTCCCCATGCTGTC; GTCACTCATCCTGCACAGGCAGAGCCACATCCCTGCCGATCAGGGACGGGAGAAGCTCCAGACCAAGACC; CTGGCTGCCGAGTCCTTGGAACAGAAGGCCTTCCTGGCCTTGCTGGGCCTGCAGCACACCAAAGACGTCA; AGCCTGCCCCGGCGGAGGAGCTCCTGCCGGACGACAACCAAGCAATACAGCTCCAGACACTTAAGTACCA; GCTACCTCAGGAGCCTGGCTGCCCCACCGTGCTGAGTGTGTCTCCTCTTGACGCTGCTTCTTTAGGTGGC; TCTCTGACAGTCCTCCCAGCTACCAAGGAGAATATGAAGCATCTGTCCCTGCAGCCCTTCCAGAAGGGCT; TCATCATCCAGCCAGACAGTAGCATTGTGGTTAAGCCTATTTCAGGAGAGTCGGCCATTGAGCTGGCAGA; TATCCAACAGATTCTAAAGATGGCAGCTTCCGCTCCTCCGCAGATCAGTCTTCCGCCACTTTCCAAGGCC; CCTGCCACCCCGCTGCAGGCGATTTTCAAGCACATGCCTCCTTTGAAGCCAAAGCCCTTGGTCACACCCC; GGACAGTGGTAGCCGCCTCCACGCCCCCACCTCTCATCAACGCCCAGCAGGCATCTCCCGGTTGTATCAG; CCCCAGCCTTCCTCCACAGTCCCTGAAGTTCCTCAAGGGGTCGGTGGAGGCAGTGTCCAATGTTCATCTG; CTTCAGTCCAAGTCTGGGATCCAGCCAAGCACCACCACACAGCTCTTCCTGCAGCAAGCTGGAGTGGAGT; TGCCGGGCCAGCCCGAGATGAAGACACAGCTGGAACAAGAGAGTATCATTGAGGCCCTGCTGCCCCTCAA; CATGGAGGCGAAGATCAAGCAGGAGATAACAGAAGGTGACCTCAAGGCCATCATGACAGGCCCCAGTGGC; AAGAAGACCCCCGCCATGCGCAAGGTGCTCTACCCCTGCCGCTTTTGCAACCAGGTGTTTGCTTTCTCTG; GAGTCCTGCGAGCCCACGTTCGCTCCCACCTGGGCATCTCACCCTACCAATGCAACATCTGTGACTATAT; TGCTGCAGACAAAGCCGCCCTGATCCGTCACATCCGCACACACAGCGGGGAACGGCCTTACATCTGCAAG; ATCTGCCACTACCCATTCACGGTCAAAGCCAACTGTGAGCGGCACCTGCGCAAGAAGCACCTCAAGGCCA; CCAGGAAGGACATTGAGAAGAACATAGAGTACGTGAGCAGCCCCACCGCAGAGCTGGTGGACGCCTTCTG; CGCCCCAGAGACCGTGTGCAGACTGTGCGGGGAGGATCTGAAGCACTACCGAGCTCTACGCATCCACATG; CGCACACACTGCAGCCGGGGCCTGGGCGGCTGCCACAAAGGCCGGAAACCCTTCGAGTGCAAGGAATGCA; ACGCTCCCTTCGTGGCCAAGCGCAACTGCATCCATCACATCCTCAAGCAGCACCTGCACGTGCCCGAGAA; GGACATCGAGAGCTACGTGCTTGCCACCAACAGTGGCCTCGGCCCCGCGGACACGCCCACGGATGCCGCT; TCCAGAGGAGAAGAGGGCAGCTGTGTCACTTTCGCGGAGTGCAAGCCCCTCGCCACTTTCCTGGAGCCCC; AAAATGGCTTTCTTCACTCGAGCCCCACCCAGCCCCTGCCTTCCCACATCTCTGTCAAGCTGGAGCCAGC; CAGCAGCTTTGCCATGGACTTCAACGAACCCCTTGACTTTTCGCAGAAAGGCCTGGCACTGGTCCAAGTG; AAGCAGGAAAATGTTTCCTCCTTGCTGACGTCTTCCTCCTCCTCTGCCCTCTATGACTGCTCCATGGAGC; CCATTGACCTGTCCATCCCCAAGAGCGTCAAGAAAGGAGACAAGGACACAGTTGTTCCCAGTGACGCCAA; GAAACCGGAACCGGAAGCTGGGCAAGCCGAGCCGCTCTCACCCCGCCCACCACCCTGCCCTACCTTGTCA; GTGACTGTGGAGCCCAAGGGGAGCCTGGAAACCCCCACAGGCACGGTGGTGGCCGTCACCACAGCTGCCA; AGCTAGAGCCCCACACTCAGCCCCTCCAGGGCTCCGTGCAGCTGGCTGTCCCCATCTACTCCCCCGCGCT; CGTCAGCAATACTCCCCTCTTGGGCAATTCTGCTGCCCTCTTGAACAACCCAGCCTTGCTTCGGCCCTTA; CGGCCAAAGCCCCCCCTCCTCTTGCCAAAGCCCTCGATGACAGAGGAGCTGCCGCCACTGGCCTCCATCG; CCCAGATCATTTCTTCCGTGTCCTCGGCCCCTACTCTGCTGAAAACGAAGGTAGCTGACCCTGGACCATC; GATCACCAGCAGTAACACTGTGGCCACAGACAGCCCAGGAAGCTCCATCCCCAAAGCTGCCGCCACCCCC; ACTGACACCACAAGCTCTAAAGAATCCAGTGAGCCACCCCCTGCAGCCAGCAGCCCTGAGGAAGCCTTGC; CTACTGAACAAGGGCCGGCTGCCACTTCCTCGTCGAGGAAGAGGGGCAGGAAAAGGGGACTGAGAAACCG; GCCCCTCCCCAACAGCAGTGCTGTGGACCTGGACTCCAGTGGGGAGTTTGCTAGCATCGAGAAGATGCTG; GCCACCACAGATACCAACAAGTTCAGTCCCTTTCTGCAGACTGCAGAGGATGACACTCAGGAAGAGGTGG; CTGGAGCCCCTGCCGACCAGCATGGGCCCGCTGATGAGGAGCAAGGTAGCCCCGCAGAAGACAGGCTGCT; GAGAGCAAAGCGGAACTCCTATGCCAACTGCCTGCAAAAGATCAACTGTCCCCACTGTCCCCGGGTCTTC; CCTTGGGCCAGCTCCCTGCAGCGGCACATGCTTACACACACTGGTAAGAAGGCCCTCACGGCTCACCAGG; CGGTGAGTCTTGAAAGGAAAGAGTAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177868
• Insert Size: 3876 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001177868.1, NP_001171339.1
• RefSeq Size: 7198 bp
• RefSeq ORF: 3876 bp
• Locus ID: 68750
• UniProt ID: Q3UH06
• Cytogenetics: 13 A3.3
• Summary: Transcription factor that binds specifically to the RAS-responsive elements (RRE) of gene promoters. May be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. Represses the angiotensinogen gene. Negatively regulates the transcriptional activity of AR. Potentiates the transcriptional activity of NEUROD1 (By similarity). Binds specifically to the allelic variant of the CDKN2A promoter present in Balb/c mice, which leads to a down-regulation of CDKN2A expression in this strain, and, as a consequence, to an elevated susceptibility to pristane-induced tumors.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 2. Variants 2-4 encode the functional protein.