Sh3pxd2a (NM_001164717) Mouse Untagged Clone

SKU MC223542

Sh3pxd2a (untagged) - Mouse SH3 and PX domains 2A (Sh3pxd2a), transcript variant 2, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Sh3pxd2a
• Synonyms: 2310014D11Rik; AA589508; AI256723; AI413738; C230050L11; EG329070; Fish; Gm5098; Sh3md1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223542 representing NM_001164717; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCTCGCCTACTGCGTGCAAGATGCCACCGTGGTGGACGTGGAGAAGCGGAGGAGCCCCTCTAAACACT; ATGTATACATTATCAACGTGACCTGGTCTGACTCCACCTCCCAGACTATCTACCGGAGGTACAGCAAGTT; CTTCGACCTGCAGATGCAGCTTCTGGATAAGTTTCCTATTGAAGGTGGCCAGAAGGATCCGAAGCAAAGG; ATTATTCCCTTTCTTCCAGGCAAGATCCTCTTCCGGAGAAGCCACATCCGCGACGTGGCTGTGAAGAGAC; TAAAGCCCATCGATGAATACTGCAGGGCGCTTGTCCGGCTGCCGCCCCACATTTCACAGTGTGACGAAGT; CTTCCGGTTCTTTGAGGCACGGCCTGAGGATGTCAACCCTCCAAAAGAAGACTATGGCAGTTCCAAGAGG; AAATCAGTGTGGTTGTCCAGCTGGGCTGAGTCTCCCAAGAAGGACGTGACAGGTGCCGACACCAACGCCG; AGCCCATGATCCTGGAACAGTACGTGGTGGTGTCCAACTATAAGAAACAAGAGAACTCGGAGCTGAGCCT; CCAGGCCGGGGAGGTGGTAGATGTCATCGAGAAGAACGAAAGCGGCTGGTGGTTTGTGAGCACATCTGAA; GAGCAAGGTTGGGTCCCCGCCACCTACTTGGAGGCCCAGAATGGCACACGAGACGACTCGGACATCAACA; CCTCCAAGACTGGGGAAGAAGAGAAGTATGTCACTGTGCAGCCCTACACCAGCCAGAGCAAAGACGAGAT; CGGCTTCGAGAAGGGTGTCACCGTGGAGGTGATTCGAAAGAACCTGGAAGGCTGGTGGTACATCAGGTAC; CTCGGCAAAGAGGGTTGGGCACCAGCGTCCTACCTGAAGAAGGCCAAGGATGACCTGCCGACCCGGAAGA; AGAACCTGGCGGGTCCGGTGGAGATCATAGGGAACATTATGGAGATCAGCAACCTTCTCAACAAGAAGGC; ATCTGGGGATAAGGAGGCTCCGGCTGAAGGCGAGGGATCCGAGGCCCCCATCACCAAGAAAGAGATCAGC; TTACCGATCCTCTGCAACGCCTCCAATGGCAGCGCCTTGGCCATTCCCGAGAGGACCACATCCAAGCTAG; CCCAGGGCTCCCCAGCTGTGGCCAGGATCGCCCCTCAGAGGGCCCAGATCAGCTCCCCAAATCTGAGGAC; AAGACCTCCCCCGCGCAGAGAATCCAGCCTGGGGTTCCAGCTGCCAAAGCCGCCAGAGCCCCCTTCTGTT; GAGGTAGAATACTACACCATTGCTGAATTCCAGTCCTGCATTTCTGACGGGATCAGCTTTCGAGGCGGAC; AGAAGGCAGAGGTCATCGACAAGAACTCGGGTGGTTGGTGGTACGTGCAGATCGGGGAGAAGGAGGGCTG; GGCCCCAGCCTCATACATTGACAAGCGCAAGAAACCCAACCTCAGCCGCCGAACCAGCACTCTGACGCGG; CCCAAGGTGCCGCCACCTGCGCCCCCCAGCAAGCCTAAGGAGGCCGAGGAGAATCCTGTGGGTGCCTGTG; AGAGCCAGGGCTCCCCACTGAAGGTTAAATACGAGGAACCCGAGTATGACGTCCCTGCCTTTGGCTTTGA; CTCAGAGCCCGAGATGAATGAAGAGCCTTCAGGGGACAGAGGTTCAGGTGACAAGCATCCCGCCCAGCCC; CGAAGGATCTCGCCTGCCTCTTCCCTGCAACGGGCCCATTTCAAGGTGGGTGAGTCTTCTGAGGACGTGG; CCCTGGAAGAGGAGACCATCTATGAGAATGAGGGCTTCAGGCCATACACAGAAGACACCCTGTCTGCCAG; AGGCTCCTCTGGGGACAGTGACTCCCCTGGGAGCTCCTCTTTGTCCCTTGCCGTGAAAAACTCCCCTAAA; TCAGATTCCCCCAAATCCTCATCACTCCTAAAGCTCAAAGCAGAGAAGAATGCCCAGGCAGAACTGGGGA; AAAACCAGTCCAACATCTCCTTCTCCTCCTCTGTCACCATCAGCACCACCTGTTCCTCCTCCTCATCCTC; GTCCTCCTTGTCCAAGAACAATGGTGACCTGAAACCACGTTCTGCCTCAGATGCAGGTATCCGTGACACC; CCTAAGGTTGGGACCAAGAAAGATCCTGATGTGAAGGCCGGGCTGGCCTCCTGCGCCCGAGCCAAGCCAT; CCGTGAGACCAAAGCCAGTCCTGAACCGAGCGGAGTCTCAAAGCCAGGAGAAGATGGATATTAGTTCCTT; ACGGCGCCAGCTGAGGCCCACAGGCCAGCTCCGGGGGGGCCTCAAGGGCTCTAGGAGTGAGGACTCAGAG; CTGCCTCCACAGATGGCTTCTGAGGGATCCAGGCGAGGTTCTGCGGACATCATCCCTCTCACGGCCACCA; CTCCCCCGTGTGTCCCCAAAAAGGAATGGGAAGGGCAAGGCGCCACCTACGTGACGTGCAGCGCCTATCA; GAAGGTCCAGGACTCGGAGATCAGCTTCCCCGAAGGCGCCGAGGTGCACGTGCTGGAGAAGGCGGAAAGT; GGGTGGTGGTACGTGAGGTTTGGGGAGCTGGAGGGCTGGGCTCCTTCCCACTACTTGGTGGCCGAGGAGA; ACCAGCAACCTGACACAGCTAGCAAAGAGGGAGACACAGGAAAGAGCTCGCAGAACGAGGGCAAGTCAGA; CAGCCTGGAAAAGATTGAGAAGCGTGTGCAGGCGCTCAACACTGTGAACCAGAGCAAGAGGGCCACCCCA; CCCATCCCCTCGAAGCCTCCCGGGGGCTTCGGCAAGACCTCGGGCACCGTAGCGGTGAAGATGAGGAACG; GGGTCCGGCAAGTGGCCGTCAGGCCCCAATCTGTGTTTGTGTCTCCGCCACCCAAGGACAACAACCTGTC; CTGTGCCCTTCGGAGGAACGAGTCGCTAACGGCCACCGACAGCCTCAGAGGTGTCCGCAGGAACTCCTCC; TTTAGCACCGCACGGTCAGCAGCCGCTGAGGCCAAGGGCCGCCTGGCCGAGCGGGCTGCCAGCCAGGGCT; CAGAATCGCCCCTGCTGCCTACCCAGCGCAAAGGCATCCCTGTCTCCCCCGTGCGTCCCAAGCCCATAGA; GAAGTCCCAGTTTATCCACAACAACCTCAAGGATGTGTACATCTCGATTGCAGACTATGAGGGGGACGAA; GAGACGGCTGGCTTCCAGGAGGGGGTGTCCATGGAGGTGCTGGAGAAGAACCCCAATGGCTGGTGGTACT; GCCAGATCCTGGATGAGGTGAAGCCCTTCAAGGGCTGGGTACCCTCCAACTACCTTGAGAAGAAGAACTA; A; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164717
• Insert Size: 3291 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001164717.1, NP_001158189.1
• RefSeq Size: 10380 bp
• RefSeq ORF: 3291 bp
• Locus ID: 14218
• UniProt ID: O89032
• Cytogenetics: 19 C3
• Summary: Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.