Ghsr (NM_177330) Mouse Untagged Clone

CAT#: MC212500

Ghsr (untagged) - Mouse growth hormone secretagogue receptor (Ghsr), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ghsr
• Synonyms: C530020I22Rik; Ghsr1a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212500 representing NM_177330
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAACGCGACGCCCAGCGAGGAGCCGGAGCCTAACGTCACGCTGGACCTGGACTGGGACGCTTCTC
  CCGGCAACGACTCACTCTCTGACGAACTGCTGCCACTGTTCCCCGCGCCGCTGCTGGCGGGCGTCACTGC
  CACCTGCGTGGCGCTCTTCGTGGTGGGCATCTCGGGCAACCTGCTCACCATGCTGGTGGTGTCCCGCTTC
  CGCGAGCTGCGCACCACCACCAACCTCTACCTATCCAGCATGGCCTTCTCCGATCTGCTCATCTTCCTGT
  GCATGCCGCTGGACCTCGTCCGCCTCTGGCAGTATCGGCCCTGGAACTTCGGCGACCTGCTCTGCAAACT
  CTTCCAGTTTGTCAGCGAGAGCTGCACCTACGCCACGGTCCTCACCATCACCGCGCTGAGCGTCGAGCGC
  TACTTCGCCATCTGCTTCCCGCTGCGGGCCAAGGTGGTGGTCACCAAGGGCCGTGTGAAGCTGGTCATCC
  TTGTCATTTGGGCCGTGGCCTTCTGCAGCGCGGGGCCCATCTTCGTGCTGGTGGGCGTGGAGCACGAGAA
  CGGCACAGATCCCCGGGACACCAACGAGTGCCGCGCCACCGAGTTCGCTGTGCGCTCTGGGCTGCTCACC
  GTGATGGTATGGGTGTCGAGCGTCTTCTTCTTCCTGCCGGTCTTCTGCCTCACTGTGCTCTACAGTCTCA
  TCGGGAGGAAGCTGTGGCGGAGGCGCGGCGACGCGGCGGTGGGCTCCTCGCTCAGGGACCAGAACCACAA
  ACAGACAGTGAAGATGCTTGCTGTGGTGGTGTTTGCTTTCATCCTCTGCTGGCTGCCCTTCCACGTGGGA
  AGATATCTGTTTTCCAAGTCTTTCGAGCCTGGCTCTCTGGAGATCGCGCAGATCAGTCAGTACTGCAACC
  TGGTGTCCTTTGTCCTCTTCTACCTCAGCGCTGCCATCAACCCCATTCTCTACAACATCATGTCCAAGAA
  GTACCGGGTGGCCGTGTTCAAACTTCTAGGATTTGAATCCTTCTCCCAGAGAAAGCTTTCCACTCTGAAG
  GATGAGAGTTCCCGGGCCTGGACAAAGTCGAGCATCAATACATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177330
• Insert Size: 1095 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC120672, AAI20673
• RefSeq Size: 1188 bp
• RefSeq ORF: 1095 bp
• Locus ID: 208188
• UniProt ID: Q99P50
• Cytogenetics: 3 A3
• Gene Summary: Receptor for ghrelin, coupled to G-alpha-11 proteins. Stimulates growth hormone secretion. Binds also other growth hormone releasing peptides (GHRP) (e.g. Met-enkephalin and GHRP-6) as well as non-peptide, low molecular weight secretagogues (e.g. L-692,429, MK-0677, adenosine) (By similarity).[UniProtKB/Swiss-Prot Function]