Atg12 (NM_026217) Mouse Untagged Clone

CAT#: MC203435

Atg12 (untagged) - Mouse autophagy-related 12 (yeast) (Atg12), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atg12
• Synonyms: 4931423H11Rik; A330058M13Rik; Apg12l; Atg12l
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC070470
  AAGATGTCGGAAGATTCAGAGGTTGTGCTGCAGCTCCCCTCGGCTCCTGTAGGAGCCGGCGGCGAGAGCC TTCCAGAGCTCTCCCCGGAAACAGCCACCCCAGAGCCCCCGTCCTCGGCTGCAGTTTCGCCCGGAACGGA GGAACCTCCCGGAGACACCAAGAAAAAAATTGACATCCTGCTGAAGGCTGTAGGAGACACTCCTATAATG AAAACAAAGAAATGGGCTGTGGAGCGAACCCGGACCATCCAAGGACTCATTGACTTCATCAAAAAGTTCC TTAAACTGGTGGCCTCGGAACAGTTGTTTATTTATGTGAATCAGTCCTTTGCCCCTTCCCCAGACCAAGA AGTTGGAACTCTATATGAGTGTTTTGGCAGTGATGGTAAACTGGTCCTGCATTACTGCAAATCCCAGGCA TGGGGATGAGCCAGAAATGAACAACTTGATATTTTCACAGAAGAAAAAAAATCAGAGACATTTCGGTGCT GTGGGAAGAGACTTACTATCAAATATTCAGCATGAATCTGTTAGGACCTGTGTTTATGTATAAGAAAACA TCATAGAGTATATAGACTAATGAATGAGATTTGTGTCACCATAAAATCATCATGAGAGATTGACGTTAGA GTGTGCCATTACTCCCACACACTCCTGTTCCAGGTCACGTTAGAAAGGAGACTGAAGTTGTATGTGGATT GCAGGTCTCAGGATGACTGAAGGCATTTACTTGGTTGGCACTATGTTAATTCCGTGATAAGAACTAATTT ACTGAAGGGTTTGTGTTTTGAGGTAATTTAGTTTTTTTGCTTATTCAGGGGACCTCTATTTGGGATATAG AATGTTTACATTTATCTGTATTGCTCTGTAAAAAATAGTGCACTGATCTAAGTTGTCCGGTAGAGGGCAG CCAGTTGGCAAAAAGGCTTGACAGTTTTCATGGCTTTGCCAGGTGATTGGGGGCAAGACTGTTGAACCTA ATTTCTAGTTAATGTGTAATCCGGAAAGCAGCTGTGTTGACACACTGGAGGATGTGCATAGTTTAAAATT AATCATGCAGGGACACAGTTTTACAAATTGTGAGCAGATGAGTCTCAGCTCTTCAGTCCTGTCATTTCTG GTTCTTGCGCCTCATCCTGGGCATTGTGATCCATACCTGCTGTCTCAGCGCTCAGGACACCTGAGCAGGA AGAGTGAACCAGGAGTCCACAACTAGACTGCACTGCAAAAGAACTTATCCCAAAAAGATTCTAGAGCCCT ACCCAGTGTGGGGACCACGCAGCACCAACTTACCCATCTCCCAACCCTCACTTCTCGTCTCTATGTGCTT TTAGTTTGGGTTTTGTTTTGGTTTTTTTGTTTTGTTTTGTTTTTTTTCCAGGCCTGGGGATCATTCCCCT TGCTTGTGCACTCAAGGCATGGGCTTTGCTGAGCTGCATCTCTCCAGCATTCATTGAGTGTTCATAATGT ATTTCCCAAATTGAAAGTATTCAGAGCTAATGGTTGAAAACTAGTATCCAATTTTATGTGTATTCCGTGC CATCACATACACACAAAAAGAGTGCAGCTGTCACTGGTGATGTTAGCCTGGCTATTGCTTGAGCTAACAT TTGACCTTTTTCTTACATATTTTATCTCAAACTTTTTTTTGAAGCTGGGTCTCACTATGTAGATCTGCCT AGCCTGGAACTTGCAGAGATCAGTGTGTCTGTCTATGCCTCCAAAGTGCTGAGATGAAGGGATAGCCCAC CTCACTCAGCCCCACAGCAGTCTTAAATTCCAACCAGAAGCACACATGGCAGCACTCCTAGCATTGAGGA AGTGGAAACAAGCAGACTGAAAGTTTAAGACTGTGTGGGCTACAGAGTGAGATTGTCTCACCTCTGGGGG AAAAATTACAGTAAAATGCTACTCTGTTGTTGTCCTGCTTTTGAGATTGACTGTGTCACTCAGGCTGACC TGCCTCAGGCTCCGGGGCTTGCAGCTGTGCACCACTGCATCTGGCTCCTTTTTAACTCTTTAGCTAGAGT GTGCTCTAGAGGAGCCTCAGCCATGTGAGCCATGGAAGAAGCTGTGGAACAACACGGTGCAGTGCTGACC ACTTGTCTTCTGCGCAAGAGTGTGAATTGTGGACATGCCCTTCCTCAGCATGCCTTGCAGTGAGGTCATG CAGGGAATAGTCACAGAGCACTGTGGACAGTGGCTTATGCACAGTACAGCCTTAGGAAAACACCTTATAT CTTGAAAACTGGGCCGTATTATTTATGAACAATGTCAGTGGGAAAGAAAAAAGATGTGTTCTAGCTTTGC CTGGTAATTGGTGACTGAAAGAACATAGTAGTAGATATTTACAGGAGAAGTATGTTGAATTTATTGTTGG CTGGTATTCATTCGAGATGACAGAATGCTTATTACTTCTATGAAATCAAAAATAAACTTGAATTTGAAAC ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_026217
• Insert Size: 426 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC070470, AAH70470
• RefSeq Size: 2490 bp
• RefSeq ORF: 426 bp
• Locus ID: 67526
• UniProt ID: Q9CQY1
• Cytogenetics: 18 C
• Gene Summary: Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes.[UniProtKB/Swiss-Prot Function]