Dnmt3a (NM_007872) Mouse Untagged Clone

SKU MC200622

Dnmt3a (untagged) - Mouse DNA methyltransferase 3A (Dnmt3a), transcript variant 1, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Dnmt3a
• Synonyms: MmuIIIA
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC007466 sequence for NM_007872; CCCACGCGTCCGCCCACGCGTCCGCGCCGCGGCAGCAGGGCGAGCCGGGCCGCCCGACCCGCCGGGCCAC ACGGCAGAGCCGCCTGAAGCCCAGCGCTGAGGCTGCACTTTTCCGAGGGCTTGACATCAGGGTCTATGTT TAAGTCTTAGCTCTTGCTTACAAAGACCACGGCAATTCCTTCTCTGAAGCCCTCGCAGCCCCACAGCGCC CTCGCAGCCCCAGCCTGCCGCCTACTGCCCAGCAATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCC TCTCTGGAGCGGGAGGATGATCGAAAGGAAGGAGAGGAACAGGAGGAGAACCGTGGCAAGGAAGAGCGCC AGGAGCCCAGCGCCACGGCCCGGAAGGTGGGGAGGCCTGGCCGGAAGCGCAAGCACCCACCGGTGGAAAG CAGTGACACCCCCAAGGACCCAGCAGTGACCACCAAGTCTCAGCCCATGGCCCAGGACTCTGGCCCCTCA GATCTGCTACCCAATGGAGACTTGGAGAAGCGGAGTGAACCCCAACCTGAGGAGGGGAGCCCAGCTGCAG GGCAGAAGGGTGGGGCCCCAGCTGAAGGAGAGGGAACTGAGACCCCACCAGAAGCCTCCAGAGCTGTGGA GAATGGCTGCTGTGTGACCAAGGAAGGCCGTGGAGCCTCTGCAGGAGAGGGCAAAGAACAGAAGCCGACC AACATCGAATCCATGAAAATGGAGGGCTCCCGGGGCCGACTGCGAGGTGGCTTGGGCTGGGAGTCCAGCC TCCGTCAGCGACCCATGCCAAGACTCACCTTCCAGGCAGGGGACCCCTACTACATCAGCAAACGGAAACG GGATGAGTGGCTGGCACGTTGGAAAAGGGAGGCTGAGAAGAAAGCCAAGGTAATTGCAGTAATGAATGCT GTGGAAGAGAACCAGGCCTCTGGAGAGTCTCAGAAGGTGGAGGAGGCCAGCCCTCCTGCTGTGCAGCAGC CCACGGACCCTGCTTCTCCGACTGTGGCCACCACCCCTGAGCCAGTAGGAGGGGATGCTGGGGACAAGAA TGCTACCAAAGCAGCCGACGATGAGCCTGAGTATGAGGATGGCCGGGGCTTTGGCATTGGAGAGCTGGTG TGGGGGAAACTTCGGGGCTTCTCCTGGTGGCCAGGCCGAATTGTGTCTTGGTGGATGACAGGCCGGAGCC GAGCAGCTGAAGGCACTCGCTGGGTCATGTGGTTCGGAGATGGCAAGTTCTCAGTGGTGTGTGTGGAGAA GCTCATGCCGCTGAGCTCCTTCTGCAGTGCATTCCACCAGGCCACCTACAACAAGCAGCCCATGTACCGC AAAGCCATCTACGAAGTCCTCCAGGTGGCCAGCAGCCGTGCCGGGAAGCTGTTTCCAGCTTGCCATGACA GTGATGAAAGTGACAGTGGCAAGGCTGTGGAAGTGCAGAACAAGCAGATGATTGAATGGGCCCTCGGTGG CTTCCAGCCCTCGGGTCCTAAGGGCCTGGAACCACCAGAAGAAGAGAAGAATCCTTACAAGGAAGTTTAC ACCGACATGTGGGTGGAGCCTGAAGCAGCTGCTTACGCCCCACCCCCACCAGCCAAGAAACCCAGAAAGA GCACAACAGAGAAACCTAAGGTCAAGGAGATCATTGATGAGCGCACAAGGGAGCGGCTGGTGTATGAGGT GCGCCAGAAGTGCAGAAACATCGAGGACATTTGTATCTCATGTGGGAGCCTCAATGTCACCCTGGAGCAC CCACTCTTCATTGGAGGCATGTGCCAGAACTGTAAGAACTGCTTCTTGGAGTGTGCTTACCAGTATGACG ACGATGGGTACCAGTCCTATTGCACCATCTGCTGTGGGGGGCGTGAAGTGCTCATGTGTGGGAACAACAA CTGCTGCAGGTGCTTTTGTGTCGAGTGTGTGGATCTCTTGGTGGGGCCAGGAGCTGCTCAGGCAGCCATT AAGGAAGACCCCTGGAACTGCTACATGTGCGGGCATAAGGGCACCTATGGGCTGCTGCGAAGACGGGAAG ACTGGCCTTCTCGACTCCAGATGTTCTTTGCCAATAACCATGACCAGGAATTTGACCCCCCAAAGGTTTA CCCACCTGTGCCAGCTGAGAAGAGGAAGCCCATCCGCGTGCTGTCTCTCTTTGATGGGATTGCTACAGGG CTCCTGGTGCTGAAGGACCTGGGCATCCAAGTGGACCGCTACATTGCCTCCGAGGTGTGTGAGGACTCCA TCACGGTGGGCATGGTGCGGCACCAGGGAAAGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAA GCATATCCAGGAGTGGGGCCCATTCGACCTGGTGATTGGAGGCAGTCCCTGCAATGACCTCTCCATTGTC AACCCTGCCCGCAAGGGACTTTATGAGGGTACTGGCCGCCTCTTCTTTGAGTTCTACCGCCTCCTGCATG ATGCGCGGCCCAAGGAGGGAGATGATCGCCCCTTCTTCTGGCTCTTTGAGAATGTGGTGGCCATGGGCGT TAGTGACAAGAGGGACATCTCGCGATTTCTTGAGTCTAACCCCGTGATGATTGACGCCAAAGAAGTGTCT GCTGCACACAGGGCCCGTTACTTCTGGGGTAACCTTCCTGGCATGAACAGGCCTTTGGCATCCACTGTGA ATGATAAGCTGGAGCTGCAAGAGTGTCTGGAGCACGGCAGAATAGCCAAGTTCAGCAAAGTGAGGACCAT TACCACCAGGTCAAACTCTATAAAGCAGGGCAAAGACCAGCATTTCCCCGTCTTCATGAACGAGAAGGAG GACATCCTGTGGTGCACTGAAATGGAAAGGGTGTTTGGCTTCCCCGTCCACTACACAGACGTCTCCAACA TGAGCCGCTTGGCGAGGCAGAGACTGCTGGGCCGATCGTGGAGCGTGCCGGTCATCCGCCACCTCTTCGC TCCGCTGAAGGAATATTTTGCTTGTGTGTAAGGGACATGGGGGCAAACTGAAGTAGTGATGATAAAAAAG TTAAACAAACAAACAAACAAAAAACAAAACAAAACAATAAAACACCAAGAACGAGAGGACGGAGAAAAGT TCAGCACCCAGAAGAGAAAAAGGAATTTAAAGCAAACCACAGAGGAGGAAAACGCCGGAGGGCTTGGCCT TGCAAAAGGGTTGGACATCATCTCCTGAGTTTTCAATGTTAACCTTCAGTCCTATCTAAAAAGCAAAATA GGCCCCTCCCCTTCTTCCCCTCCGGTCCTAGGAGGCGAACTTTTTGTTTTCTACTCTTTTTCAGAGGGGT TTTCTGTTTGTTTGGGTTTTTGTTTCTTGCTGTGACTGAAACAAGAGAGTTATTGCAGCAAAATCAGTAA CAACAAAAAGTAGTAGCCTTGGAGAGGAAAGGGAGAGAGGGAAAATTCTATAAAAACTTAAAATATTGGT TTTTTTTTTTTTCCTTTTCTATATATCTCTTTGGTTGTCTCTAGCCTGATCAGATAGGAGCACAAACAGG AAGAGAATAGAGACCCTCGGAGGCAGAGTCTCCTCTCCCACCCCCGAGCAGTCTCAACAGCACCATTCCT GGTCATGCAAAACAGAACCCAACTAGCAGCAGGGCGCTGAGAGAACACCACACCAGACACTTTTCTACAG TATTTCAGGTGCCTACCACACAGGAAACCTTGAAGAAAACCAGTTTCTAGAAGCCGCTGTTACCTCTTGT TTACAGTTTATATATATATGATAGATATGAGATATATATATATAAAAGGTACTGTTAACTACTGTACATC CCGACTTCATAATGGTGCTTTCAAAACAGCGAGATGAGCAAAGACATCAGCTTCCGCCTGGCCCTCTGTG CAAAGGGTTTCAGCCCAGGATGGGGAGAGGGGAGCAGCTGGAGGGGGTTTTAACAAACTGAAGGATGACC CATATCACCCCCCACCCCTGCCCCATGCCTAGCTTCACCTGCCAAAAAGGGGCTCAGCTGAGGTGGTCGG ACCCTGGGGAAGCTGAGTGTGGAATTTATCCAGACTCGCGTGCAATAACCTTAGAATATGAATCTAAAAT GACTGCCTCAGAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_007872
• Insert Size: 2727 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC007466, AAH07466
• RefSeq Size: 4094 bp
• RefSeq ORF: 2727 bp
• Locus ID: 13435
• UniProt ID: O88508
• Cytogenetics: 12 A1.1
• Summary: This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene causes developmental defects in multiple different organ systems. There is a pseudogene for this gene located on chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1, also known as Dnmt3a1). Variants 1 and 3 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.