Gpr137b (NM_031999) Mouse Untagged Clone

SKU MC200041

Gpr137b (untagged) - Mouse G protein-coupled receptor 137B (Gpr137b), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Gpr137b
• Synonyms: AI788917; AU016435; AW546472; C80550; C80741; C730041E01; D13Abb1e; Tm7sf1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC003212 sequence for NM_031999; GCGGCGGGAGGAAGTGCGGCTTGTTTTCCCGGCTAGGCTCTGGAGCGGCGGGCGCGGCGCGATGCGCGGG TACCCGGGAGCGAACGGCTGCGAGCCCTGATGAAGCTCGAGCAGCCCCAGCCTGATGGAGGCGCCTCCGT GGGAGCCGGTGCGCAATGACTCCCTGCCTCCCACGCTGAGCCCCGCGGTGCCGCCCTACGTGAAGCTCGG CCTCACCGCGGTCTACACCGTCTTCTACGCGCTCCTCTTCGTGTTCATCTATGCGCAGCTCTGGCTGGTG CTGCGCTACCGTCACAAGCGGCTCAGCTACCAGAGCGTCTTCCTCTTCCTTTGCCTCTTCTGGGCCTCGC TGCGTACCGTGCTCTTCTCCTTTTACTTCCGAGACTTCGTGGCAGCCAACTCGTTCAGCCCCTTCGTCTT CTGGCTGCTCTACTGCTTCCCCGTGTGTCTACAGTTCTTCACCCTCACGCTCATGAACTTGTACTTCACG CAGGTGATTTTCAAGGCCAAGTCAAAATATTCTCCAGAGCTACTCAAATACCGGTTACCCCTCTACCTGG CCTCACTTTTCATCAGCCTCGTTTTCCTGTTGGTGAATCTGACCTGTGCTGTGCTGGTGAAGACGGGAGA CTGGGACAGGAAGGTTATCGTCTCTGTGAGAGTGGCCATCAATGACACACTCTTTGTGCTGTGTGCTATC TCTCTCTCCATCTGCCTCTACAAAATCTCCAAGATGTCCCTGGCCAACATCTACTTGGAGTCAAAGGGCT CATCAGTGTGTCAGGTAACTGCCATTGGTGTCACCGTCATCTTGCTCTACACCTCTCGGGCCTGCTACAA CCTGTTCATCTTGTCATTTTCTCAGATCAAGAACGTCCATTCCTTTGATTATGACTGGTACAATGTATCC GACCAGGCAGATCTGAAGAGCCAGCTGGGTGACGCCGGCTACGTAGTGTTTGGCGTGGTGCTTTTCGTGT GGGAGCTCCTACCCACCACCTTGGTGGTTTATTTCTTCCGAGTCAGAAATCCCACGAAGGATCTTACCAA TCCTGGGATGGTCCCCAGCCATGGATTCAGTCCCAGATCTTACTTCTTTGACAACCCCCGAAGATATGAC AGTGATGATGACCTTGCCTGGAACATTGCCCCTCAGGGACTTCAGGGAAGTTTTGCTCCAGACTACTGTG ATTGGGGACAACAAAATAACAGCTTCCTGGCACAAGCAGGAACTTTGCATCAAGACTCCACTTTGGATCC AGACAAAGCAAGCCAAGGGTAGCAGCAGCTGACACAGCCCTATGGAAGAGTTCTCTGTTGAAAGCCTTCA GCCAGACAGACCGGATGACAGCTGAGTTGCTAAGGCAGTTTTCCTTAGGAAACAGAACTCTAGTTTTTGC TATAGCTTTCTCATGGCTCCACAGGGCTAAGCAATAATTTAGAGCAATAAACTCTTTAGTACTAGCAGAG AATCTGGCTATTTCAGTGGGTATAATTTAAACTTATAAAAGAGGTTCTGTACTTTTATAAAGATGTATTT TATATAACTTAAATACTAATGCTAAAGTATACTAGGTTTTTCCTTGATTGTTAATTGCAACGTATGTTGT AGTTTGCACAGACTTTCATGCATAATTCACTTTAAAACGTATAGAATACGTGGTCTAATAGTTTAAAGCT TTGGGGAAAGTTTCCACAAATCTTACCTCTGAAGGTCCCTCTTGTGAGTGCCACGTGGTGGGCTCCTTTC ACCGCCACTCAAGCATTCCAAGTTCAGGAGAAGCAGAGTACCATGGTCTGTACGTAACAGGCTCAACAGC AGCAGCAGCAGCAGCAGCAGCAGCAGCAATTTCTCGTAAACTCTGTCCTAAGCCTGGTCCTTCTTCATCT GAAAGCACTACTACAAGCACTCCAGTAACAAGTGGATACTGTTAAGATGTAGTTGCTGACACTATTAAAC CTCCTCTGCTGTGTGTGTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_031999
• Insert Size: 1158 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC003212, AAH03212
• RefSeq Size: 1993 bp
• RefSeq ORF: 1158 bp
• Locus ID: 83924
• UniProt ID: Q8BNQ3
• Cytogenetics: 13 5.24 cM
• Summary: Lysosomal integral membrane protein that regulates the localization and activity of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Interacts with Rag GTPases and increases the lysosomial localization and activity of Rag GTPases and thereby regulates mTORC1 translocation and activity in lysosome. Involved in the regulation of lysosomal morphology and autophagy (By similarity). Acts also as a negative regulator of osteoclast activity (PubMed:31173907). Involved in interleukin-4-induced M2 macrophage polarization (PubMed:30595385).[UniProtKB/Swiss-Prot Function]