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pCMV6-AC-mRFP Mammalian Expression Vector

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SKU
PS100041
pCMV6-AC-mRFP, mammalian vector with C-terminal mRFP, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag mRFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal mRFP (monomeric) tag.
  • mRFP is a monomeric red (orange) fluorescent protein generated from the wild-type RFP from sea anemone Entacmaea quadricolor. It possesses bright fluorescence with excitation/emission maxima at 555 and 584 nm, respectively. mRFP is about three times brighter than mCherry protein, which makes it the brightest monomeric red fluorescent protein available so far.
    mRFP is mainly intended for protein labeling and FRET applications. It can also be used for cell and organelle labeling and for tracking the promoter activity.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal mRFP-tagging vector, PS100049.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • RFP_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

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Simultaneous membrane binding of Annexin A4 and A5 suppresses 2D lattice formation while maintaining curvature induction
Mularski, Sønder, Heitmann et al
J Colloid Interface Sci (2021) 600, 854-864 DOI: 10.1016/j.jcis.2021.05.067
Cited by 14
AI Summary: OriGene products, specifically the turbo-GFP C-terminal tagged human ANXA4 (RG203229) and ANXA5 (RG205619) expression plasmids, were used to transiently express labeled annexins in MCF7 cells, enabling the study of their membrane localization and cooperative behavior during plasma membrane repair.
Snippet: …Expression plasmids with turbo-GFP C-terminal tag containing human ANXA4 or ANXA5 were purchased from OriGene Technologies (OriGene RG203229 and RG205619). ANXA4-tRFP;- 4. … Expression plasmids with turbo-GFP C-terminal tag containing human ANXA4 or ANXA5 were purchased from OriGene Technologies (OriGene RG203229 and RG205619). ANXA4-tRFP
Novel Nephronophthisis-associated variants reveal functional importance of MAPKBP1 dimerization for centriolar recruitment
Schönauer, Jin, Ertel et al
Kidney Int. (2020) 98 (4), 958-969 DOI: 10.1016/j.kint.2020.05.027
Cited by 14
AI Summary: OriGene's pCMV6-AN-mRFP plasmid was used to generate N-terminally RFP-tagged MAPKBP1 constructs, enabling the study of protein localization and interactions in this research.
Snippet: To generate N-terminally RFP-tagged MAPKBP1, pCMV6-AN-mRFP (OriGene Technologies, Rockville, Maryland, US) and pcDNA3.1D_JNKBP1/2xDDK-His-TOPO were digested with FastDigest MssI and FastDigest HindIII (FD1344, Thermo Fisher Scientific, US)
Tsg101 positively regulates P62-Keap1-Nrf2 pathway to protect hearts against oxidative damage
Deng, Essandoh, Wang et al
Redox Biol (2020) 32, 101453 DOI: 10.1016/j.redox.2020.101453
Cited by 68
AI Summary: OriGene products Myc-Tsg101 (MR206104) and myc-vector (PS100001) were used to transfect neonatal rat cardiomyocytes, enabling the authors to analyze the role of Tsg101 in autophagy flux and its impact on the p62/Keap1/Nrf2 pathway during oxidative stress in heart cells.
Snippet: … The in vitro autophagy flux analysis was conducted in neonatal rat cardiomyocytes which were transfected with mRFP-GFP-LC3 (Addgene plasmid # 21074) and Myc-Tsg101 or myc-vector (Origene, MR206104, PS100001) for 48 h. … The yellow dots represent combined;- The in vitro autophagy flux analysis was conducted in neonatal rat cardiomyocytes which were transfected with mRFP-GFP-LC3 (Addgene plasmid # 21074) and Myc-Tsg101 or myc-vector (Origene, MR206104, PS100001) for 48 h. Then, these transfected cells were
Structure-activity relationship study of estrogen receptor down-regulators with a diphenylmethane skeleton
Nanjyo, Ohgane, Yoshioka et al
Bioorg. Med. Chem. (2019) 27 (10), 1952-1961 DOI: 10.1016/j.bmc.2019.03.042
AI Summary: OriGene’s pCMV-AC-mRFP vector was used to prepare a blank vector for fusing FLAG and mRFP tags to proteins of interest, facilitating the study’s development of tagged constructs for analyzing estrogen receptor down-regulators.
Snippet: ;First, a blank vector for fusing FLAG tag and mRFP tag (pCMV-AC-FLAGmRFP) to a protein of interest was prepared from pCMV-AC-mRFP (OriGene)
Inhibition of p70 S6 kinase activity by A77 1726 induces autophagy and enhances the degradation of superoxide dismutase 1 (SOD1) protein aggregates
Sun, Mu, Jiang et al
Cell Death Dis (2018) 9 (3), 407 DOI: 10.1038/s41419-018-0441-0
AI Summary: OriGene contributed to this study by providing the RFP-LC3 expression vector (pmRFP-LC3), which was used to visualize and analyze autophagosome formation during experiments investigating the effects of S6K1 inhibition on autophagy and SOD1 protein aggregate degradation.
Snippet: . The SOD1-GFP and SOD1G93A-GFP expression vectors were prepared by inserting a GFP gene downstream of SOD1 in a pcDNA3.1 vector. The expression vector encoding RFP-LC3 (pmRFP-LC3) was purchased from OriGene Technologies, Inc. (Rockville, MD). The NSC34 cell line was complete DMEM medium supplemented with 10% fetal bovine serum, streptomycin and penicillin, and L-glutamine. Western blot Cells gro...
FigureFigureFigure
Annexin A4 and A6 induce membrane curvature and constriction during cell membrane repair
Boye, Maeda, Pezeshkian et al
Nat Commun (2017) 8 (1), 1623 DOI: 10.1038/s41467-017-01743-6
AI Summary: OriGene’s I k B 3 (Cat# TP760703) product was used in this study to investigate the molecular mechanisms underlying cell membrane repair, specifically in experiments analyzing the roles of annexin A4 and A6 in membrane curvature and constriction.
Snippet: Article.;I k B 3 OriGene Cat# TP760703 ABCD1 Dana Farber Harvard cDNA Clone ID: HsCD00039863
FigureFigureFigure
Showing 1-6 of 11 papers.
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