pLenti-P2A-Puro Lentiviral Gene Expression Vector

CAT#: PS100109

pLenti-P2A-Puro, tag free Lenti vector with P2A-Puro, 10 ug

Product images

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Specifications

Product Data

• E. coli Selection: Chloramphenicol
• Mammalian Cell Selection: Puromycin
• Features:

  This Lenti vector is a third generation lentiviral vector.

  • All OriGene's TrueORF clones can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF (no stop codon for P2A-Puro) cloned in this vector will be expressed as native protein, no tag.
  • P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli. selection: Chloramphenicol (34 ug/ml)
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • P2A-DDK_R Primer can be used to sequence the target sequence after cloning.
• Schematic of the multiple cloning sites: