pLenti-P2A-Puro Lentiviral Gene Expression Vector
E. coli Selection
Mammalian Cell Selection
This Lenti vector is a third generation lentiviral vector.
All OriGene's TrueORF clones can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process. The ORF (no stop codon for P2A-Puro) cloned in this vector will be expressed as native protein, no tag. P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells. A brief lentiviral packaging protocol is provided.
E. Coli. selection: Chloramphenicol (34 ug/ml)
V2_F Primer can be used to sequence the target sequence after cloning.
P2A-DDK_R Primer can be used to sequence the target sequence after cloning.
Schematic of the multiple cloning sites
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks