pLenti-EF1a-C-Myc-DDK-IRES-Puro Lentiviral Gene Expression Vector

CAT#: PS100085

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pLenti-EF1a-C-Myc-DDK-IRES-Puro, Lenti Vector with EF1 alpha promoter and bicistronic IRES-Puro, 10ug



Special Discount: 20% off this product. Use code: "Lenti20".

USD 850.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (3)
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Forward primer to sequence targets cloned in expression vectors.
    • 1 nmol

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Reverse primer to sequence targets cloned in expression vectors.
    • 1 nmol

USD 71.00

Specifications

Product Data
C-Tag Myc-DDK
E. coli Selection Chloramphenicol
Mammalian Cell Selection Puromycin
Features

This Lenti vector is a third generation of lentiviral vector.

  • All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The expression of the cloned ORF is driven by EF1a, a constitutive promoter as an alternative of CMV promoter. The latter could be silenced in certain cellular environment.
  • The ORF cloned in this vector can be expressed as a tagged protein with c-terminal Myc-DDK tags.
  • IRES-Puro is within the Lentiviral packaging cassette and would allow Puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided
  • EF51 Primer can be used to sequence the target sequence after cloning.
  • IRES100 Primer can be used to sequence the target sequence after cloning.
Schematic of the multiple cloning sites
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.