pLenti-EF1a-C-Myc-DDK-IRES-Puro Lentiviral Gene Expression Vector

CAT#: PS100085

pLenti-EF1a-C-Myc-DDK-IRES-Puro, Lenti Vector with EF1 alpha promoter and bicistronic IRES-Puro, 10ug

Product images

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Specifications

Product Data

• C-Tag: Myc-DDK
• E. coli Selection: Chloramphenicol
• Mammalian Cell Selection: Puromycin
• Features:

  This Lenti vector is a third generation of lentiviral vector.

  • All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The expression of the cloned ORF is driven by EF1a, a constitutive promoter as an alternative of CMV promoter. The latter could be silenced in certain cellular environment.
  • The ORF cloned in this vector can be expressed as a tagged protein with c-terminal Myc-DDK tags.
  • IRES-Puro is within the Lentiviral packaging cassette and would allow Puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided
  • EF51 Primer can be used to sequence the target sequence after cloning.
  • IRES100 Primer can be used to sequence the target sequence after cloning.
• Schematic of the multiple cloning sites: