pLenti-EF1a-C-mGFP Lentiviral Gene Expression Vector
pLenti-EF1a-C-mGFP, Lenti Vector with EF1 alpha promoter and C-terminal mGFP tag, 10ug
E. coli Selection
This Lenti vector is a third generation of lentiviral vector.
All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process. The ORF cloned in this vector can be expressed as a tagged protein with a C-terminal mGFP tag. The expression of the cloned ORF is driven by EF1a, a constitutive promoter as an alternative of CMV promoter. The latter could be silenced in certain cellular environment. A brief lentiviral packaging protocol is provided . E. Coli selection: Chloramphenicol(34ug/ml)
EF51 Primer can be used to sequence the target sequence after cloning.
mGFP_R Primer can be used to sequence the target sequence after cloning. Download full vector sequence
Schematic of the multiple cloning sites
TurboFectin Transfection Reagent (1 mL in 1 vial)
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks