ABCA1 Human shRNA Plasmid Kit (Locus ID 19)

CAT#: TL315036

ABCA1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL315036A virus into HEK293 cells. TL315036A virus was prepared using lenti-shRNA TL315036A and TR30037 packaging kit.

• 

  GFP signal was observed under microscope at 48 hours after transduction of TL315036A virus into HEK293 cells. TL315036A virus was prepared using lenti-shRNA TL315036A and TR30037 packaging kit.

• 

  GFP signal was observed under microscope at 48 hours after transduction of TL315036B virus into HEK293 cells. TL315036B virus was prepared using lenti-shRNA TL315036B and TR30037 packaging kit.

• 

  GFP signal was observed under microscope at 48 hours after transduction of TL315036B virus into HEK293 cells. TL315036B virus was prepared using lenti-shRNA TL315036B and TR30037 packaging kit.

• 

  GFP signal was observed under microscope at 48 hours after transduction of [TL315036C] virus into HEK293 cells. [TL315036C] virus was prepared using lenti-shRNA [TL315036C] and TR30037 packaging kit.

• 

  GFP signal was observed under microscope at 48 hours after transduction of [TL315036C] virus into HEK293 cells. [TL315036C] virus was prepared using lenti-shRNA [TL315036C] and TR30037 packaging kit.

• GFP signal was observed under microscope at 48 hours after transduction of [TL315036D] virus into HEK293 cells. [TL315036D] virus was prepared using lenti-shRNA [TL315036D] and TR30037 packaging kit.

• GFP signal was observed under microscope at 48 hours after transduction of [TL315036D] virus into HEK293 cells. [TL315036D] virus was prepared using lenti-shRNA [TL315036D] and TR30037 packaging kit.

Show all 8 images

Specifications

Product Data

• Locus ID: 19
• Synonyms: ABC-1; ABC1; CERP; HDLCQTL13; HDLDT1; HPALP1; TGD
• Vector: pGFP-C-shLenti
• E. coli Selection: Chloramphenicol (34 ug/ml)
• Mammalian Cell Selection: Puromycin
• Format: Lentiviral plasmids
• Kit Components: ABCA1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 19). 5µg purified plasmid DNA per construct
  29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• RefSeq: BC034824, NM_005502, NM_005502.1, NM_005502.2, NM_005502.3, BC146856, NM_005502.4
• UniProt ID: O95477
• Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in both alleles of this gene cause Tangier disease and familial high-density lipoprotein (HDL) deficiency. [provided by RefSeq, Sep 2019]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).