RBM16 (SCAF8) Human shRNA Plasmid Kit (Locus ID 22828)

SKU TL309916

SCAF8 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Product Data

• Locus ID: 22828
• Synonyms: RBM16
• Vector: pGFP-C-shLenti
• E. coli Selection: Chloramphenicol (34 ug/ml)
• Format: Lentiviral plasmids
• Components: SCAF8 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 22828). 5µg purified plasmid DNA per construct; 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• RefSeq: NM_001286188, NM_001286189, NM_001286194, NM_001286199, NM_014892, NM_014892.1, NM_014892.2, NM_014892.3, NM_014892.4, NM_001286199.1, NM_001286194.1, NM_001286189.1, NM_001286188.1, BC070071, BC032728, BM976977, NM_014892.5
• UniProt ID: Q9UPN6
• Summary: Anti-terminator protein required to prevent early mRNA termination during transcription (PubMed:31104839). Together with SCAF4, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins (PubMed:31104839). Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation (PubMed:31104839). Independently of SCAF4, also acts as a positive regulator of transcript elongation (PubMed:31104839).[UniProtKB/Swiss-Prot Function]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Shipping: Ambient