p21 (CDKN1A) Human shRNA Plasmid Kit (Locus ID 1026)

SKU TL305469

CDKN1A - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Product Data

• Locus ID: 1026
• Synonyms: CAP20; CDKN1; CIP1; MDA-6; P21; p21CIP1; SDI1; WAF1
• Vector: pGFP-C-shLenti
• E. coli Selection: Chloramphenicol (34 ug/ml)
• Format: Lentiviral plasmids
• Components: CDKN1A - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 1026). 5µg purified plasmid DNA per construct; 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• RefSeq: NM_000389, NM_001220777, NM_001220778, NM_001291549, NM_078467, NR_037150, NR_037151, NR_037152, NM_078467.1, NM_078467.2, NM_000389.1, NM_000389.2, NM_000389.4, NM_001220777.1, NM_001220778.1, NM_001291549.1, BC000312, BC000312.2, BC013967, BC013967.2, BC000275, BC001935, NM_001220777.2, NM_078467.3, NM_001220778.2, NM_001291549.3, NM_000389.5
• UniProt ID: P38936
• Summary: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Shipping: Ambient