CD33 Human shRNA Plasmid Kit (Locus ID 945)

SKU TG314092

CD33 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided

Product Data

• Locus ID: 945
• Synonyms: p67; SIGLEC-3; SIGLEC3
• Vector: pGFP-V-RS
• E. coli Selection: Kan
• Format: Retroviral plasmids
• Components: CD33 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 945). 5µg purified plasmid DNA per construct; 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
• RefSeq: NM_001082618, NM_001177608, NM_001772, NM_001772.1, NM_001772.2, NM_001772.3, NM_001082618.1, NM_001177608.1, BC028152, NM_001082618.2, NM_001177608.2
• UniProt ID: P20138
• Summary: Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:15597323, PubMed:11320212). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:28325905, PubMed:10887109). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10556798, PubMed:10206955, PubMed:10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323).[UniProtKB/Swiss-Prot Function]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Shipping: Ambient