MCL1 Human shRNA Plasmid Kit (Locus ID 4170)
MCL1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector
|Product Name||MCL1 Human shRNA Plasmid Kit (Locus ID 4170)|
|Synonyms||bcl2-L-3; BCL2L3; EAT; Mcl-1; MCL1-ES; mcl1/EAT; MCL1L; MCL1S; TM|
|Kit Components||MCL1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 4170). 5µg purified plasmid DNA per constructNon-effective 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.|
|RefSeq||NM_001197320, NM_021960, NM_182763|
|Summary||This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]|
|shRNA Design||These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service|
|Performance Guaranteed||OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
|The use of this RNAi has been cited in the following citations:|
ER Stress-Mediated Upregulation of miR-29a Enhances Sensitivity to Neuronal Apoptosis
,Nolan, K;Walter, F;Tuffy, LP;Poeschel, S;Gallagher, R;Haunsberger, S;Bray, I;Stallings, RL;Concannon, CG;Prehn, JH;,
Eur. J. Neurosci. Jan 2016,PubMed ID 26750440
Modulation of Mcl-1 sensitizes glioblastoma to TRAIL-induced apoptosis
,Murphy, AC;Weyhenmeyer, B;Noonan, J;Kilbride, SM;Schimansky, S;Loh, KP;KÃ¶gel, D;Letai, AG;Prehn, JH;Murphy, BM;,
Apoptosis Nov 2013,PubMed ID 24213561
Survivin Inhibitor YM-155 Sensitizes Tumor Necrosis Factor– Related Apoptosis-Inducing Ligand-Resistant Glioma Cells to Apoptosis through Mcl-1 Downregulation and by Engaging the Mitochondrial Death Pathway
,Daniel R. Premkumar, Esther P. Jane, Kimberly A. Foster, and Ian F. Pollack,
J. Pharmacol. Exp. Ther., Aug 2013; 346: 201 - 210.,PubMed ID 23740602
YM-155 Potentiates the Effect of ABT-737 in Malignant Human Glioma Cells via Survivin and Mcl-1 Downregulation in an EGFR-Dependent Context
,Esther P. Jane, Daniel R. Premkumar, Joseph D. DiDomenico, Bo Hu, Shi-Yuan Cheng, and Ian F. Pollack,
Mol. Cancer Ther., Mar 2013; 12: 326 - 338.