LIM Kinase 1 (LIMK1) Human shRNA Plasmid Kit (Locus ID 3984)

SKU TF320403

LIMK1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided

Product Data

• Locus ID: 3984
• Synonyms: LIMK; LIMK-1
• Vector: pRFP-C-RS
• E. coli Selection: Chloramphenicol (34 ug/ml)
• Format: Retroviral plasmids
• Components: LIMK1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 3984). 5µg purified plasmid DNA per construct; 29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
• RefSeq: NM_001204426, NM_002314, NM_016735, NM_002314.1, NM_002314.2, NM_002314.3, NM_001204426.1, BC148340, BC152982, NM_001204426.2, NM_002314.4
• UniProt ID: P53667
• Summary: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Shipping: Ambient