p16INK4A (CDKN2A) Human shRNA Plasmid Kit (Locus ID 1029)

SKU TF319880

CDKN2A - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided

Product Data

• Locus ID: 1029
• Synonyms: ARF; CDK4I; CDKN2; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4; P16INK4A; P19; P19ARF; TP16
• Vector: pRFP-C-RS
• E. coli Selection: Chloramphenicol (34 ug/ml)
• Format: Retroviral plasmids
• Components: CDKN2A - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 1029). 5µg purified plasmid DNA per construct; 29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
• RefSeq: BC015960, NM_000077, NM_001195132, NM_058195, NM_058196, NM_058197, NM_058195.1, NM_058195.2, NM_058195.3, NM_058197.1, NM_058197.2, NM_058197.3, NM_058197.4, NM_000077.1, NM_000077.2, NM_000077.3, NM_000077.4, NM_001195132.1, BC015960.2, BC021998, NM_001363763, NM_000077.5, NM_001195132.2, NM_058197.5
• UniProt ID: P42771
• Summary: This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Shipping: Ambient