BRCA1 Human shRNA Plasmid Kit (Locus ID 672)

SKU TF314440

BRCA1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided

Product Data

• Locus ID: 672
• Synonyms: BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53
• Vector: pRFP-C-RS
• E. coli Selection: Chloramphenicol (34 ug/ml)
• Format: Retroviral plasmids
• Components: BRCA1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 672). 5µg purified plasmid DNA per construct; 29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
• RefSeq: NM_007294, NM_007295, NM_007296, NM_007297, NM_007298, NM_007299, NM_007300, NM_007301, NM_007302, NM_007303, NM_007304, NM_007305, NM_007306, NR_027676, NM_007299.1, NM_007299.2, NM_007299.3, NM_007294.1, NM_007294.2, NM_007294.3, NM_007297.1, NM_007297.2, NM_007297.3, NM_007300.1, NM_007300.2, NM_007300.3, NM_007298.1, NM_007298.2, NM_007298.3, NM_007302.2, NM_007304.2, NM_007295.2, NM_007303.2, NM_007301.2, BC115037, BC012577, BC030969, BC038947, BC046142, BC062429, BC072418, BC085615, BC106745, BC106746, BC114511, BC114562, NM_007297.4, NM_007299.4, NM_007294.4
• UniProt ID: P38398
• Summary: This gene encodes a 190 kD nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The BRCA1 gene contains 22 exons spanning about 110 kb of DNA. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2020]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Shipping: Ambient