Kitl (NM_013598) Mouse Recombinant Protein

SKU TP723775

Purified recombinant protein of Mouse kit ligand (Kitl / SCF )

Product Data

• Species: Mouse
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Mouse SCF, the region of Lys26-Ala189, from gene Accession# NM_013598
• Tag: Tag Free
• Predicted MW: 18.3 kDa
• Concentration: lot specific
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 10 mM NaHPO4 pH 7.2, 0.15M NaCl
• Bioactivity: The ED50 is 15 ng/ml, corresponding to a specific activity of 6.7 x 10^4 units/mg, determined by the dose dependent stimulation of TF-1 cell proliferation.
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_038626
• Locus ID: 17311
• UniProt ID: P20826
• Cytogenetics: 10 51.4 cM
• RefSeq Size: 5449
• RefSeq ORF: 819
• Synonyms: blz; Clo; Con; contrasted; Gb; Kitlg; Mgf; SCF; SF; Sl; SLF
• Summary: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.[UniProtKB/Swiss-Prot Function]