IL10 (NM_000572) Human Recombinant Protein

SKU TP723723

Purified recombinant protein of Human interleukin 10 (IL10)

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Human IL-10, the region of Ser19-Asn178, from gene Accession# NM_000572
• Tag: Tag Free
• Predicted MW: 18.6 kDa
• Concentration: lot specific
• Purity: >95%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 1 x PBS
• Bioactivity: The ED50 is 0.1 - 0.3 ng/ml corresponding to a specific activity of 3.3 - 10 x 10^6 units/mg, determined by the dose dependent inhibition of IFNg production by PHA activated PBMC.
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_000563
• Locus ID: 3586
• UniProt ID: P22301
• Cytogenetics: 1q32.1
• RefSeq Size: 1629
• RefSeq ORF: 534
• Synonyms: CSIF; GVHDS; IL-10; IL10A; TGIF
• Summary: The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, May 2020]
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Allograft rejection, Asthma, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway