Map4k2 (NM_009006) Mouse Recombinant Protein

SKU TP517131

Purified recombinant protein of Mouse mitogen-activated protein kinase kinase kinase kinase 2 (Map4k2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR217131 representing NM_009006; Red=Cloning site Green=Tags(s); ; MALLRDVSLQDPRDRFELLQRVGAGTYGDVYKARDTVTSELAAVKIVKLDPGDDISSLQQEITILRECRH; PNVVAYIGSYLRNDRLWICMEFCGGGSLQEIYHATGPLEERQIAYVCREALKGLHHLHSQGKIHRDIKGA; NLLLTLQGDVKLADFGVSGELTASVAKRRSFIGTPYWMAPEVAAVERKGGYNELCDVWALGITAIELGEL; QPPLFHLHPMRALMLMSKSSFQPPKLRDKTRWTQNFHHFLKLALTKNPKKRPTAERLLQHPFTTQHLPPA; LLTQLLDKASDPHLGTLSPEDSELETHDMFPDTIHSRSHHGPAERTPSEIQFHQVKFGAPRRKETDPLNE; PWEEEWTLLGKEELSGSLLQSVQEALEERSLTIRPALELQELDSPDDAIGTIKRAPFLGLPHTESTSGDN; AQSCSPGTLSAPPAGPGSPALLPTAWATLKQQEDRERSSCHGLPPTPKVHMGACFSKVFNGCPLQIHAAV; TWVHPVTRDQFLVVGAEEGIYTLNLHELHEDTMEKLISQRCSWLYCVNNVLLSLSGKSTHIWAHDLPGLF; EQRRLQHQAPLSIPTNRITQRIIPRRFALSTKIPDTKGCLQCRVVRNPYTGSTFLLAALPASLLLLQWYE; PLQKFLLLKNFSSPLPSPAGMLEPLVLDGKELPQVCVGAEGPEGPGCRVLFHVLPLEAGLTPDILIPPEG; IPGSAQQVIQVDRDTVLVSFERCVRIVNLQGEPTAALAPELTFDFTIETVVCLQDSVLAFWSHGMQGRSL; DTNEVTQEITDETRIFRVLGAHRDIILESIPTDNPGAHSNLYILTGHQSSY; ; SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 91.7 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_033032
• Locus ID: 26412
• UniProt ID: Q61161
• Cytogenetics: 19 A
• RefSeq Size: 2466
• RefSeq ORF: 2463
• Synonyms: AI385662; BL44; GCK; Rab8ip
• Summary: Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed:8643544). Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extent of the p38 MAPKs signaling pathway (By similarity). Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin (By similarity). To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation (By similarity). The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8 (By similarity). Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation (By similarity). Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3 (By similarity). May play a role in the regulation of vesicle targeting or fusion (By similarity).[UniProtKB/Swiss-Prot Function]