Cyp4x1 (NM_001003947) Mouse Recombinant Protein

SKU TP515201

Purified recombinant protein of Mouse cytochrome P450, family 4, subfamily x, polypeptide 1 (Cyp4x1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR215201 representing NM_001003947; Red=Cloning site Green=Tags(s); ; MEASWLETRWARPLHLALVFCLALVLMQAMKLYLRRQRLLRDLSPFPGPPAHWLLGHQKFLQEDNMETLD; EIVKKHPCAFPCWVGPFQAFFYIYDPDYAKIFLSRTDPKMQYLHQLLTPCIGRGLLNLDGPRWFQHRCLL; TPAFHQDILKPCVDTMAHSVKVMLDKWEKMWTTQETTIEVFEHINLMTLDIIMKCAFGQETNCQINGTYE; SYVKATFELGEIISSRLYNFWHHHDIIFKLSPKGHCFQELGKVIHQYTEKIIQDRKKILKNQVKQDDTQT; SQIFLDIVLSAQAEDERAFSDADLRAEVNTFMWAGHDASAASISWLLYCLALNPEHQDRCRTEIRSILGD; GSSITWEQLDEMSYTTMCIKETLRLIPPVPSISRELSKPLTLPDGHSLPAGMTVVLSIWGLHHNPAVWND; PKVFDPLRFTKENSDQRHPCAFLPFSSGPRNCIGQQFAMLELKVAIALILLHFQVAPDLTRPPAFSSHTV; LRPKHGIYLHLKKLLEC; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 59 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_001003947
• Locus ID: 81906
• UniProt ID: Q6A152
• Cytogenetics: 4 D1
• RefSeq Size: 1524
• RefSeq ORF: 1521
• Synonyms: A230025G20; Cyp4a28-ps; CYPIVX1; CYP_a
• Summary: A cytochrome P450 monooxygenase that selectively catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide, potentially modulating endocannabinoid signaling. Has no hydroxylase activity toward various fatty acids, steroids and prostaglandins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).[UniProtKB/Swiss-Prot Function]