Nub1 (NM_016736) Mouse Recombinant Protein

SKU TP509965

Purified recombinant protein of Mouse negative regulator of ubiquitin-like proteins 1 (Nub1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR209965 protein sequence; Red=Cloning site Green=Tags(s); ; MAQKKYLQAKLTQFLREDRIQLWKPPYTKENKEVGLAVKDLAKKYSERLECCENEVENIIEEIRRKAIER; GTGNEHYRTTGIATIEVFLPPRLRKHDKKSLLETRLHVTGRDLRCQIAETFGFQENYIKIVINKKQLQLG; KSLEEQGVTHNVKAMVLELKQSEEDVRKNLQLEEEEQNEAELKERRIQRTKRGLEILAERAEMVVDPETM; PYLDIANQTGRSLRIPPAERKALMLAMGYHEKGRAFLKRKEYGIALPCLLDADRYFCECKELLDTVDNYA; VLQLDIVWCYFRLEQLECLDDAEKKLNLAQKCFKNCYGENHQRLVHIKGNCGKEKVLFLRLYLLQGIQNY; HSGNGEEAREYLNKARQLFKELYIDPSKVHNLLQLGFTAQEARLGLRACDGNVDHAATHISNRREELAQI; RKEEKEKRRRRLENVNTLRGMGYSTQAAKQALHQARGNLDDALKVLLSNPHMWWLQDADPENNSRQASPS; QESINQLVYMGFDTVVAEAALRVFGGNVQLAAQTLAHHGGSLPPDLQFSGEDSSPTPSTSPSDSAGTSSA; STDEDMETEAVNEILEDIPEHEEDYLDSTLEDEEVIIAEYLSYVESISSAAKNN; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 70.3 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_058016
• Locus ID: 53312
• UniProt ID: P54729
• Cytogenetics: 5 A3
• RefSeq Size: 3455
• RefSeq ORF: 1842
• Synonyms: 4931404D21Rik; 6330412F12Rik; BS4; NY-REN-18
• Summary: Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation (By similarity).[UniProtKB/Swiss-Prot Function]