Pomgnt2 (NM_153540) Mouse Recombinant Protein

SKU TP509097

Purified recombinant protein of Mouse protein O-linked mannose beta 1,4-N-acetylglucosaminyltransferase 2 (Pomgnt2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR209097 representing NM_153540; Red=Cloning site Green=Tags(s); ; MHLSAVFNALLVSVLAAVLWKHVRLREHAATLEEELALGQQSLDPVLGLKIDYPKALQILMEGGTHMVCT; GRTHTDRICRFKWLCYSNEAEEFIFFHGNSSVMLPNLGSRRFQPALLDLSTVEDHNAQYFNFVELPAAAL; RFMPKPVFVPDVALIANRFNPDNLMHVFHDDLLPLFYTLRQFPGLAQEARLFFMEGWGEGAHFDLYKLLS; PKQPLLRAQLKTLGRLLCFSHAFVGLSKVTTWYQYGFVQPQGPKANILVSGNEIRQFTRFMTERLNVSHA; GAPLGEEYILVFSRTQNRLILNEAELLLELAQEFQMKTVTVSLEDHTFADVVRLVSNASMLVSMHGAQLV; TALFLPRGATVVELFPYAVNPDHYTPYKTLATLPGMDLQYVAWRNMIRENTVTHPERPWDQGGITHLDRA; EQARILQSREVPRHLCCRNPEWLFRIYQDTRVDIPSLMQSIRRVVKGRPGPRRQRWAISLYPGKVREARC; QASVQGATEARLSVSWQIPWNLKYLKVREVKYEVWLQEQGENTYVPYMLTLQNHTFTENIKPFTTYLVWV; RCIFNRSLLGPFADVLVCST; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 66.7 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_705768
• Locus ID: 215494
• UniProt ID: Q8BW41
• Cytogenetics: 9 F4
• RefSeq Size: 2369
• RefSeq ORF: 1740
• Synonyms: Ago61; C85492; Gtdc2
• Summary: O-linked mannose beta-1,4-N-acetylglucosaminyltransferase that transfers UDP-N-acetyl-D-glucosamine to the 4-position of the mannose to generate N-acetyl-D-glucosamine-beta-1,4-O-D-mannosylprotein (By similarity). Involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (PubMed:24256719).[UniProtKB/Swiss-Prot Function]