Mgrn1 (NM_029657) Mouse Recombinant Protein

SKU TP508529

Purified recombinant protein of Mouse mahogunin, ring finger 1 (Mgrn1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR208529 protein sequence; Red=Cloning site Green=Tags(s); ; MGSILSRRIAGVEDIDIQANSAYRYPPKSGNYFASHFFMGGEKFDTPHPEGYLFGENMDLNFLGSRPVQF; PYVTPAPHEPVKTLRSLVNIRKDSLRLVRYKEDADSPTEDGEKPRVLYSLEFTFDADARVAITIYCQAVE; ELVNGVAVYSCKNPSLQSETVHYKRGVSQQFSLPSFKIDFSEWKDDELNFDLDRGVFPVVIQAVVDEGDV; VEVTGHAHVLLAAFEKHVDGSFSVKPLKQKQIVDRVSYLLQEIYGIENKNNQETKPSDDENSDNSSECVV; CLSDLRDTLILPCRHLCLCTSCADTLRYQANNCPICRLPFRALLQIRAVRKKPGALSPISFSPVLAQSVD; HDEHSSSDSIPPGYEPISLLEALNGLRAVSPAIPSAPLYEEITYSGISDGLSQASCPLAGLDRIMESGLQ; KGKTQSKSPDSTLRSPSFPIHEEDEEKLSEDSDAPLPPSGVELVLRESSSPESFGTEEGDEPSLKQGSRV; PSIDDVLQDGSPQHHGCSQPVPPADIYLPALGPESCSVGIEE; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 58.5 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_083933
• Locus ID: 17237
• UniProt ID: Q9D074
• Cytogenetics: 16 2.48 cM
• RefSeq Size: 3290
• RefSeq ORF: 1596
• Synonyms: 2610042J20Rik; md; mKIAA0544; nc
• Summary: E3 ubiquitin-protein ligase. Mediates TSG101 monoubiquitination at multiple sites. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination (By similarity). Acts also as a negative regulator of hedgehog signaling (PubMed:29290584).[UniProtKB/Swiss-Prot Function]