Osteopontin (SPP1) (NM_001040060) Human Recombinant Protein
Purified recombinant protein of human secreted phosphoprotein 1 (SPP1), transcript variant 3, with C-terminal MYC/DDK tag, secretory expressed in HEK293 cells, 20ug
Specifications
Product Data | |
Description | Purified recombinant protein of human secreted phosphoprotein 1 (SPP1), transcript variant 3, with C-terminal MYC/DDK tag, secretory expressed in HEK293 cells, 20ug |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | Recombinant protein was produced with TrueORF clone, RC210592. Click on the TrueORF clone link to view cDNA and protein sequences. |
Tag | C-Myc/DDK |
Predicted MW | 30.6 kDa |
Concentration | >50 ug/mL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol |
Bioactivity | Cell treatment (PMID: 29959420) |
Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
Storage | Store at -80°C. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Reference Data | |
RefSeq | NP_001035149 |
Locus ID | 6696 |
Refseq Size | 1560 |
Cytogenetics | 4q22.1 |
Refseq ORF | 861 |
Synonyms | BNSP; BSPI; ETA-1; OPN |
Summary | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
Protein Families | Druggable Genome, Secreted Protein |
Protein Pathways | ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway |
Citations (1)
The use of this Proteins has been cited in the following citations: |
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Osteopontin isoforms differentially promote arteriogenesis in response to ischemia via macrophage accumulation and survival
,Lee, GS;Salazar, HF;Joseph, G;Lok, ZSY;Caroti, CM;Weiss, D;Taylor, WR;Lyle, AN;,
Lab. Invest. 2018
,PubMed ID 29959420
[SPP1]
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Resources
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC400422 | SPP1 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 110.00 |
|
LC421887 | SPP1 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 110.00 |
|
LC424632 | SPP1 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 110.00 |
|
LY400422 | Transient overexpression lysate of secreted phosphoprotein 1 (SPP1), transcript variant 3 |
USD 360.00 |
|
LY421887 | Transient overexpression lysate of secreted phosphoprotein 1 (SPP1), transcript variant 1 |
USD 360.00 |
|
LY424632 | Transient overexpression lysate of secreted phosphoprotein 1 (SPP1), transcript variant 2 |
USD 360.00 |
|
PH304803 | SPP1 MS Standard C13 and N15-labeled recombinant protein (NP_001035147) |
USD 2,260.00 |
|
PH305118 | SPP1 MS Standard C13 and N15-labeled recombinant protein (NP_000573) |
USD 2,260.00 |
|
PH310592 | SPP1 MS Standard C13 and N15-labeled recombinant protein (NP_001035149) |
USD 2,260.00 |
|
TP304803 | Purified recombinant protein of Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 1 |
USD 748.00 |
|
TP305118 | Purified recombinant protein of Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 2 |
USD 748.00 |
|
TP710213 | Purified protein of Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 1, residues 17-314aa, secretory expressed with GP67 signal peptide, with C-terminal DDK tag, expressed in sf9, 20ug |
USD 425.00 |
|
TP720449 | Recombinant protein of human secreted phosphoprotein 1 (SPP1), transcript variant 2 |
USD 300.00 |
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TP723344 | Purified recombinant protein of Human secreted phosphoprotein 1 (SPP1), transcript variant 2. |
USD 140.00 |
USD 379.00
USD 350.00