LDHA (NM_005566) Human Recombinant Protein

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SKU
TP309378
Recombinant protein of human lactate dehydrogenase A (LDHA), transcript variant 1, 20 µg
  $867.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC209378 representing NM_005566
Red=Cloning site Green=Tags(s)

MATLKDQLIYNLLKEEQTPQNKITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGSL
FLRTPKIVSGKDYNVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNVVKYSPNCKLLIVSNPV
DILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHPLSCHGWVLGEHGDSSVPVWSGMNVAGVS
LKTLHPDLGTDKDKEQWKEVHKQVVESAYEVIKLKGYTSWAIGLSVADLAESIMKNLRRVHPVSTMIKGL
YGIKDDVFLSVPCILGQNGISDLVKVTLTSEEEARLKKSADTLWGIQKELQF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 36.5 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Bioactivity Higher specific activity than endogenous human LDHA: OriGene human recombinant LDHA (TP309378) was compared side-by-side with purified human liver LDH5 in a spectrophotometric pyruvate to lactate conversion assay. Activity is shown as a decrease in absorbance at 340nm over time. The activity of recombinant human LDHA is comparable to that of endogenously expressed human LDH5.
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_005557
Locus ID 3939
UniProt ID P00338
Cytogenetics 11p15.1
RefSeq Size 1661
RefSeq ORF 996
Synonyms GSD11; HEL-S-133P; LDHM; PIG19
Summary The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. provided by RefSeq, Sep 2008
Protein Categories Intracellular Proteins
Protein Families Druggable Genome
Protein Pathways Cysteine and methionine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism
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Other "LDHA" proteins (8)
SKU Description Size Price
PH309378 LDHA MS Standard C13 and N15-labeled recombinant protein (NP_005557) 10 ug
$3,360.00
LC401712 Lyophilized LDHA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC427601 Lyophilized LDHA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC431321 Lyophilized LDHA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY401712 Transient overexpression lysate of lactate dehydrogenase A (LDHA), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY427601 Transient overexpression lysate of lactate dehydrogenase A (LDHA), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY431321 Transient overexpression lysate of lactate dehydrogenase A (LDHA), transcript variant 3 (as WB positive control) 100 ug
$436.00
TP720209 Recombinant protein of human lactate dehydrogenase A (LDHA), transcript variant 2 10 ug
$340.00

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