LAP3 (NM_015907) Human Recombinant Protein
$737.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 56 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | LAP3 activity verified in a biochemical assay: Leucine aminopeptidase 3 (LAP3, TP309052) activity was measured in a fluorescent biochemical assay. LAP3 catalyzes the removal of unsubstituted N-terminal amino acids from various peptides and is most active on leucine. LAP3 activity was measured in a 100 µl reaction mixture containing 1 mM L-leucine 7-amido-4-methyl coumarin (Leu-AMC), 50 mM Tris, pH 8.0, 4 mM MgCl2, and 1 mM MnCl2. Cleavage of leucine from the AMC moiety results in a strong increase in fluorescence intensity. Fluorescence was measured over time with an excitation wavelength of 380 nm and an emission wavelength of 460 nm. The activity of the enzyme in this system remained constant over six hours. |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_056991 |
| Locus ID | 51056 |
| UniProt ID | P28838 |
| Cytogenetics | 4p15.32 |
| RefSeq Size | 2100 |
| RefSeq ORF | 1557 |
| Synonyms | HEL-S-106; LAP; LAPEP; PEPS |
| Summary | Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.UniProtKB/Swiss-Prot Function |
| Protein Categories | Enzyme: Peptidases, Intracellular Proteins |
| Protein Families | Druggable Genome, Protease |
| Protein Pathways | Arginine and proline metabolism, Glutathione metabolism, Metabolic pathways |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "LAP3" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH309052 | LAP3 MS Standard C13 and N15-labeled recombinant protein (NP_056991) | 10 ug |
$3,360.00
|
|
| LC402470 | Lyophilized LAP3 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY402470 | Transient overexpression lysate of leucine aminopeptidase 3 (LAP3) (as WB positive control) | 100 ug |
$436.00
|
|
Citations
Aminopeptidase Expression in Multiple Myeloma Associates with Disease Progression and Sensitivity to Melflufen
Miettinen, Kumari, Traustadottir et al
Cancers (Basel) (2021) 13 (7) DOI: 10.3390/cancers13071527
Miettinen, Kumari, Traustadottir et al
Cancers (Basel) (2021) 13 (7) DOI: 10.3390/cancers13071527
Cited by 55
AI Summary: OriGene products, specifically the LAP3, LTA4H, RNPEP, and ANPEP cDNA clones, were used in this study to analyze the ability of these aminopeptidases to hydrolyze melflufen, thereby elucidating their role in drug sensitivity and multiple myeloma progression.
A computational method for detection of ligand-binding proteins from dose range thermal proteome profiles
Kurzawa, Becher, Sridharan et al
Nat Commun (2020) 11 (1), 5783 DOI: 10.1038/s41467-020-19529-8
Kurzawa, Becher, Sridharan et al
Nat Commun (2020) 11 (1), 5783 DOI: 10.1038/s41467-020-19529-8
Cited by 56
AI Summary: OriGene’s recombinant LAP3 protein (NM_015907) was used to assess leucine aminopeptidase activity in vitro, enabling the validation of drug-protein interactions identified in the study.
Snippet: performed using non-moderated F-statistics. Fluorometric aminopeptidase assay LAP3 activity was determined using the Leucine Aminopeptidase Activity Assay Kit (Abcam, ab124627) and recombinant LAP3 (Origene, NM_015907). Recombinant LAP3 enzyme was dissolved in the kit assay buffer and incubated for 10 minutes at room temperature with vehicle (dimethyl sulfoxide) or 100 μM of either PCI-34051
Computational analysis of ligand dose range thermal proteome profiles
Kurzawa, Becher, Sridharan et al
bioRxiv (2020)
Kurzawa, Becher, Sridharan et al
bioRxiv (2020)
Cited by 5
Showing 1-3 of 3 papers.
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