ERG (NM_182918) Human Recombinant Protein

CAT#: TP308093

Recombinant protein of human v-ets erythroblastosis virus E26 oncogene homolog (avian) (ERG), transcript variant 1

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 Product Datasheet for 'TP308093'

USD 680


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    • 20 ug

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Specifications

Product Data
Description Recombinant protein of human v-ets erythroblastosis virus E26 oncogene homolog (avian) (ERG), transcript variant 1
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence Recombinant protein was produced with TrueORF clone, RC208093. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag C-MYC/DDK
Predicted MW 53.7 kDa
Concentration >50 ug/mL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Bioactivity Varying amounts of human ERG expressed in HEK293 cells was incubated for one hour with wild-type or mutant biotinylated oligonucleotide (1 pmole/ul) in the presence of 25 ug/ml poly dI:dC. The reaction mixture was subsequently transferred to a microplate containing 2500 Luminex beads coupled with anti-ERG monoclonal antibody 2G8. The ERG-oligo complexes were captured onto the antibody-coated beads for two hours at room temperature with shaking. The beads were then washed, and the biotin was detected with streptavidin-phycoerythrin for 30 minutes. The beads were washed again and the fluorescent intensity was read in the Luminex instrument. The wild-type oligonucleotide carried ACCGGAAGT consensus binding sequence while the mutant oligonucleotide was identical except for a 2-base mutation in the consensus binding region, ACCCCAAGT
Reference Data
RefSeq NP_891548
Locus ID 2078
Refseq Size 3055
Cytogenetics 21q22.2
Refseq ORF 1437
Synonyms erg-3; p55
Summary Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure. [UniProtKB/Swiss-Prot Function]
Protein Families
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