ERG (NM_182918) Human Recombinant Protein
SKU
TP308093
Recombinant protein of human v-ets erythroblastosis virus E26 oncogene homolog (avian) (ERG), transcript variant 1, 20 µg
$867.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 53.7 kDa |
| Concentration | >0.1 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | Varying amounts of human ERG expressed in HEK293 cells was incubated for one hour with wild-type or mutant biotinylated oligonucleotide (1 pmole/ul) in the presence of 25 ug/ml poly dI:dC. The reaction mixture was subsequently transferred to a microplate containing 2500 Luminex beads coupled with anti-ERG monoclonal antibody 2G8. The ERG-oligo complexes were captured onto the antibody-coated beads for two hours at room temperature with shaking. The beads were then washed, and the biotin was detected with streptavidin-phycoerythrin for 30 minutes. The beads were washed again and the fluorescent intensity was read in the Luminex instrument. The wild-type oligonucleotide carried ACCGGAAGT consensus binding sequence while the mutant oligonucleotide was identical except for a 2-base mutation in the consensus binding region, ACCCCAAGT ELISA capture for autoantibodies (PMID: 28191285) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_891548 |
| Locus ID | 2078 |
| UniProt ID | P11308 |
| Cytogenetics | 21q22.2 |
| RefSeq Size | 3055 |
| RefSeq ORF | 1437 |
| Synonyms | erg-3; p55 |
| Summary | This gene encodes a member of the erythroblast transformation-specific (ETS) family of transcriptions factors. All members of this family are key regulators of embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. The protein encoded by this gene is mainly expressed in the nucleus. It contains an ETS DNA-binding domain and a PNT (pointed) domain which is implicated in the self-association of chimeric oncoproteins. This protein is required for platelet adhesion to the subendothelium, inducing vascular cell remodeling. It also regulates hematopoesis, and the differentiation and maturation of megakaryocytic cells. This gene is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia. More than two dozens of transcript variants generated from combinatorial usage of three alternative promoters and multiple alternative splicing events have been reported, but the full-length nature of many of these variants has not been determined. provided by RefSeq, Apr 2014 |
| Protein Categories | Cancer: Sarcoma, Intracellular Proteins, Transciption Factors |
| Protein Families | Druggable Genome, Transcription Factors |
Reviews
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| FAQs |
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Resources
| Recombinant Protein Resources |
Other "ERG" proteins (9)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH308093 | ERG MS Standard C13 and N15-labeled recombinant protein (NP_891548) | 10 ug |
$3,360.00
|
|
| PH318892 | ERG MS Standard C13 and N15-labeled recombinant protein (NP_004440) | 10 ug |
$3,360.00
|
|
| LC401413 | Lyophilized ERG HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC403650 | Lyophilized ERG HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC427827 | Lyophilized ERG HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY401413 | Transient overexpression lysate of v-ets erythroblastosis virus E26 oncogene homolog (avian) (ERG), transcript variant 2 (as WB positive control) | 100 ug |
$665.00
|
|
| LY403650 | Transient overexpression lysate of v-ets erythroblastosis virus E26 oncogene homolog (avian) (ERG), transcript variant 1 (as WB positive control) | 100 ug |
$436.00
|
|
| LY427827 | Transient overexpression lysate of v-ets erythroblastosis virus E26 oncogene homolog (avian) (ERG), transcript variant 3 (as WB positive control) | 100 ug |
$436.00
|
|
| TP318892 | Recombinant protein of human v-ets erythroblastosis virus E26 oncogene homolog (avian) (ERG), transcript variant 2, 20 µg | 20 ug |
$867.00
|
|
Citations
Epigenome-wide impact of MAT2A sustains the androgen-indifferent state and confers synthetic vulnerability in ERG fusion-positive prostate cancer
Cacciatore, Shinde, Musumeci et al
Nat Commun (2024) 15 (1), 6672 DOI: 10.1038/s41467-024-50908-7
Cacciatore, Shinde, Musumeci et al
Nat Commun (2024) 15 (1), 6672 DOI: 10.1038/s41467-024-50908-7
Cited by 14
AI Summary: In this study, the OriGene human recombinant ERG protein (cat. TP308093) was used in microscale thermophoresis (MST) experiments to investigate protein interactions relevant to the role of MAT2A in prostate cancer.
Snippet: For MST experiments, Histidine-tagged MAT2A protein (BPS Bioscience, cat 71401) was labelled with fluorescent dye using Monolith His-Tag Labeling Kit (cat MO-L008) (NanoTemper Technologies GmbH). Human recombinant protein ERG (OriGene, cat. TP308093) or BSA was incubated with labelled MAT2A (5 nM) at room temperature for 30 minutes. Microscale thermophoresis (MST)
EZH2-induced lysine K362 methylation enhances TMPRSS2-ERG oncogenic activity in prostate cancer
Zoma, Curti, Shinde et al
Nat Commun (2021) 12 (1), 4147 DOI: 10.1038/s41467-021-24380-6
Zoma, Curti, Shinde et al
Nat Commun (2021) 12 (1), 4147 DOI: 10.1038/s41467-021-24380-6
Cited by 46
AI Summary: In this study, OriGene products including the ERG (RC221011) and EZH2 (RC212065) expression plasmids were used to investigate how EZH2-induced methylation of ERG at lysine K362 enhances the oncogenic activity of TMPRSS2-ERG in prostate cancer.
Snippet: ognosis. Discussion Aberrant expression of the transcription factor ERG produces profound changes in the transcriptome of prostate epithelial cells. However, the mechanisms underlying ERG-induced tumorigenesis and its impact on tumor progession are still unclear. In this study, we uncovered a relevant mechanism promoting progression in ERG-fusion positive prostate cancer. We found that EZH2 inter
Autoantibodies against oncogenic ERG protein in prostate cancer: potential use in diagnosis and prognosis in a panel with C-MYC, AMACR and HERV-K Gag
Rastogi, Ali, Tan et al
Genes Cancer (2016) 7 (11-12), 394-413 DOI: 10.18632/genesandcancer.126
Rastogi, Ali, Tan et al
Genes Cancer (2016) 7 (11-12), 394-413 DOI: 10.18632/genesandcancer.126
Snippet: Recombinant full length ERG protein, produced in mammalian cells upon transfection of expression plasmid DNA, was purchased from Origene (Rockville, MD). Recombinant proteins for AMACR and cMYC, produced in mammalian cells, were also purchased ... All 2 versions Cite SaveSaving
Analytical platform evaluation for quantification of ERG in prostate cancer using protein and mRNA detection methods
He, Schepmoes, Shi et al
Journal of Translational Medicine (2015) 13 (54)
He, Schepmoes, Shi et al
Journal of Translational Medicine (2015) 13 (54)
AI Summary: OriGene contributed recombinant full length ERG protein, which was used as a standard in assay development and sensitivity testing for ERG protein detection in this study.
Snippet: All cells were cultured in humidified conditions, at 37°C with 5% CO2. Sample preparation Recombinant full length ERG protein, produced in mammalian cells by exogenous expression, was purchased from Origene (Rockville, MD). ... Cite SaveSaving
Showing 1-4 of 4 papers.
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