CHMP4B (NM_176812) Human Recombinant Protein
$737.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 24.8 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_789782 |
| Locus ID | 128866 |
| UniProt ID | Q9H444 |
| Cytogenetics | 20q11.22 |
| RefSeq Size | 1664 |
| RefSeq ORF | 672 |
| Synonyms | C20orf178; CHMP4A; CTPP3; CTRCT31; dJ553F4.4; Shax1; SNF7; SNF7-2; Vps32-2; VPS32B |
| Summary | This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies. Mutations in this gene result in autosomal dominant posterior polar cataracts.provided by RefSeq, Oct 2009 |
| Protein Categories | Cytokines, Intracellular Proteins, Nervous system Diseases |
| Protein Pathways | Endocytosis |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "CHMP4B" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH307637 | CHMP4B MS Standard C13 and N15-labeled recombinant protein (NP_789782) | 10 ug |
$3,360.00
|
|
| LC406132 | Lyophilized CHMP4B HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY406132 | Transient overexpression lysate of chromatin modifying protein 4B (CHMP4B) (as WB positive control) | 100 ug |
$436.00
|
|
Citations
ADAPT identifies an ESCRT complex composition that discriminates VCaP from LNCaP prostate cancer cell exosomes
Hornung, O'Neill, Logie et al
Nucleic Acids Res. (2020) 48 (8), 4013-4027 DOI: 10.1093/nar/gkaa034
Hornung, O'Neill, Logie et al
Nucleic Acids Res. (2020) 48 (8), 4013-4027 DOI: 10.1093/nar/gkaa034
Cited by 32
AI Summary: OriGene's recombinant CHMP4B protein was used in ELONA and ELISA assays to assess the binding specificity of selected ssODNs to components of the ESCRT complex in exosome samples from prostate cancer cell lines.
Snippet: ELONA and ELISA High binding plates (Corning) were coated overnight, shak- ing at 4 ◦ C, with 10 nM of the following recombinant proteins of interest: CHMP1B (MyBioSource), CHMP2A (MyBioSource), CHMP4B (Origene), CHMP4C (Sigma- Aldrich), CPSF5 (Abcam;ELONA and ELISA High binding plates (Corning) were coated overnight, shak- ing at 4 ◦ C, with 10 nM of the following recombinant proteins of interest: CHMP1B (MyBioSource), CHMP2A (MyBioSource), CHMP4B (Origene), CHMP4C (Sigma- Aldrich), CPSF5 (Abcam), CSPF7
Showing 1-1 of 1 papers.
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