ECH1 (NM_001398) Human Recombinant Protein

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SKU
TP305622
Recombinant protein of human enoyl Coenzyme A hydratase 1, peroxisomal (ECH1), 20 µg
  $867.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC205622 protein sequence
Red=Cloning site Green=Tags(s)

MAAGIVASRRLRDLLTRRLTGSNYPGLSISLRLTGSSAQEAASGVALGEAPDHSYESLRVTSAQKHVLHV
QLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAGKMFTAGIDLMDMASDILQPKGDDVARISW
YLRDIITRYQETFNVIERCPKPVIAAVHGGCIGGGVDLVTACDIRYCAQDAFFQVKEVDVGLAADVGTLQ
RLPKVIGNQSLVNELAFTARKMMADEALGSGLVSRVFPDKEVMLDAALALAAEISSKSPVAVQSTKVNLL
YSRDHSVAESLNYVASWNMSMLQTQDLVKSVQATTENKELKTVTFSKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 35.6 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_001389
Locus ID 1891
UniProt ID Q13011
Cytogenetics 19q13.2
RefSeq Size 1276
RefSeq ORF 984
Synonyms HPXEL
Summary This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. provided by RefSeq, Jul 2008
Protein Categories Intracellular Proteins
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Other "ECH1" proteins (3)
SKU Description Size Price
PH305622 ECH1 MS Standard C13 and N15-labeled recombinant protein (NP_001389) 10 ug
$3,360.00
LC419959 Lyophilized ECH1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY419959 Transient overexpression lysate of enoyl Coenzyme A hydratase 1, peroxisomal (ECH1) (as WB positive control) 100 ug
$436.00

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