Arginase 1(ARG1) (NM_000045) Human Recombinant Protein

CAT#: TP304649

Recombinant protein of human arginase, liver (ARG1)

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 Product Datasheet for 'TP304649'

USD 680


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Size
    • 20 ug

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Specifications

Product Data
Description Recombinant protein of human arginase, liver (ARG1)
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence Recombinant protein was produced with TrueORF clone, RC204649. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag C-MYC/DDK
Predicted MW 34.6 kDa
Concentration >50 ug/mL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Bioactivity Arginase activity verified in a biochemical assay: Arginase 1 (ARG1, TP304649) activity was measured in a colorimetric biochemical assay. Arginase 1 catalyzes the conversion of arginine to ornithine and urea. After incubation of the protein in a solution containing L-arginine, the reaction is stopped, and the urea concentration is measured by a chemical reaction that produces a colored product that absorbs at 430 nm. By measuring the absorbance at 430 nm and comparing to a standard, the specific activity of this preparation of ARG1 was calculated to be approximately 10U/mg.
Unit definition: 1 unit of ARG1 converts 1 µmole of L-arginine to ornithine and urea per minute at pH 9.5 and 37ºC.
Reference Data
RefSeq NP_000036
Locus ID 383
Refseq Size 1447
Cytogenetics 6q23.2
Refseq ORF 966
Synonyms OTTHUMP00000017209; arginase 1; liver-type arginase; type I arginase; arginase, liver
Summary Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].
Protein Families
Protein Pathways
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