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Cleavage stimulation factor 2 (CSTF2) (NM_001325) Human Recombinant Protein

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SKU
TP304450L
Recombinant protein of human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa (CSTF2), 1 mg
Size:
1 mg 20 ug 100 ug
  $7,820.00
6 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC204450 protein sequence
Red=Cloning site Green=Tags(s)

MAGLTVRDPAVDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCEYQDQETA
LSAMRNLNGREFSGRALRVDNAASEKNKEELKSLGTGAPVIESPYGETISPEDAPESISKAVASLPPEQM
FELMKQMKLCVQNSPQEARNMLLQNPQLAYALLQAQVVMRIVDPEIALKILHRQTNIPTLIAGNPQPVHG
AGPGSGSNVSMNQQNPQAPQAQSLGGMHVNGAPPLMQASMQGGVPAPGQMPAAVTGPGPGSLAPGGGMQA
QVGMPGSGPVSMERGQVPMQDPRAAMQRGSLPANVPTPRGLLGDAPNDPRGGTLLSVTGEVEPRGYLGPP
HQGPPMHHVPGHESRGPPPHELRGGPLPEPRPLMAEPRGPMLDQRGPPLDGRGGRDPRGIDARGMEARAM
EARGLDARGLEARAMEARAMEARAMEARAMEARAMEVRGMEARGMDTRGPVPGPRGPIPSGMQGPSPINM
GAVVPQGSRQVPVMQGTGMQGASIQGGSQPGGFSPGQNQVTPQDHEKAALIMQVLQLTADQIAMLPPEQR
QSILILKEQIQKSTGAP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 60.8 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_001316
Locus ID 1478
UniProt ID P33240
Cytogenetics Xq22.1
RefSeq Size 2027
RefSeq ORF 1731
Synonyms CstF-64
Summary This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs. provided by RefSeq, Jul 2008
Protein Categories Growth Factors, Intracellular Proteins
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Documents
Resources

file-alt Citations

RNA Binding Protein CELF2 Regulates Signal-Induced Alternative Polyadenylation by Competing with Enhancers of the Polyadenylation Machinery
Chatrikhi, Mallory, Gazzara et al
Cell Rep (2019) 28 (11), 2795-2806.e3 DOI: 10.1016/j.celrep.2019.08.022
AI Summary: OriGene's Recombinant CstF64 protein (catalog number TP304450) was used in this study to investigate the molecular mechanisms by which CELF2 regulates alternative polyadenylation in T cells.
Snippet: FBS GIBCO 16000-044 Lipofectamine 2000 reagent Invitrogen 52887 Protein G Sepharose beads GE Healthcare 17-0618-01 Recombinant CFIm25 Abcam ab104669 Recombinant CstF64 Origene TP304450 Recombinant CELF2 Dr. Kristen W. Lynch Ajith et. al., 2016 Recombinant hnRNPL Dr. Kristen W. Lynch Chiou et. al., 2013 Recombinant hnRNPK Dr. Kristen W. Lynch T
FigureFigureFigure
Showing 1-1 of 1 papers.
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