HSP70-1A (HSPA1A) (NM_005345) Human Recombinant Protein

CAT#: TP300270

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Recombinant protein of human heat shock 70kDa protein 1A (HSPA1A)

Size: 20 ug 100 ug 1 mg

  View other "HSPA1A" proteins (4)

USD 867.00

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    • 20 ug

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Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence
>RC200270 representing NM_005345
Red=Cloning site Green=Tags(s)


Tag C-Myc/DDK
Predicted MW 69.9 kDa
Concentration >50 ug/mL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
Bioactivity Higher specific activity than E. coli derived HSP70: Origene human recombinant Hsp70 (TP300270) was compared side-by-side with E. coli derived Hsp70 in a firefly luciferase refolding assay. Percentage of refolding is relative to an identical load of non-denatured luciferase in the reaction. The human cell produced Hsp70 is approximately 30% more active than the bacterial produced Hsp70
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq NP_005336
Locus ID 3303
UniProt ID P08107, P0DMV8, P0DMV9, A8K5I0, B3KTT5
Cytogenetics 6p21.33
Refseq Size 2383
Refseq ORF 1923
Synonyms HEL-S-103; HSP70-1; HSP70-1A; HSP70-2; HSP70.1; HSP70.2; HSP70I; HSP72; HSPA1
Summary This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]
Protein Pathways Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Prion diseases, Spliceosome


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