Rat IgG (Fc Fragment) Rat Protein

SKU RA107

Rat IgG Fc Fragment rat protein, 1 mg

Product Data

• Species: Rat
• Protein Source: Serum
• Concentration: lot specific
• Purity: This product was prepared from normal serum Delipidation, Salt Fractionation, Ion Exchange Chromatography followed by papain digestion and extensive dialysis against the buffer.
• Conjugation: Biotin
• Buffer: State: Lyophilized purified Ig fraction.; Buffer System: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.; Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC).; Molar Ratio: 10-20 BAC molecules per Rat IgG F(c) molecule.; Presentation Label: Biotin
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Preparation: Lyophilized purified Ig fraction.
• Recommended Dilution: Suitable for use in Immunoblotting, ELISA, Immunohistochemistry, Immuno-microscopy as well as other antibody based assays using streptavidin or avidin conjugates.; Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rat IgG, anti-Rat IgG F(c) and anti-Rat Serum.; No reaction was observed against anti-Rat IgG F(ab')2 or anti-Papain.
• Protein Description: Biotin Conjugated Rat IgG F(c) fragment
• Storage: Store (undiluted) at 2-8°C prior to restoration.; For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.; Avoid cycles of freezing and thawing.
• Stability: Shelf life: one year from despatch.; Centrifuge product if not completely clear after standing at room temperature.; Dilute only prior to immediate use.
• Shipping: Blue Ice

Reference Data

• Synonyms: Rat Immunoglobulin G
• Protein Families: anti-Rat IgG, anti-Rat IgG F(c) and anti-Rat Serum.<br />No reaction was observed against anti-Rat IgG F(ab')2 or anti-Papain., ELISA, Immuno-microscopy as well as other antibody based assays using streptavidin or avidin conjugates.<br />Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, Immunohistochemistry, Suitable for use in Immunoblotting