14-3-3 sigma (SFN) (NM_006142) Human Mass Spec Standard

SKU PH304045

SFN MS Standard C13 and N15-labeled recombinant protein (NP_006133)

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: [RC204045]
• Predicted MW: 27.8 kDa
• Protein Sequence:
  Protein Sequence

  >RC204045 protein sequence; Red=Cloning site Green=Tags(s); ; MERASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERNLLSVAYKNVVGGQRAAWRVLSSIEQKSN; EEGSEEKGPEVREYREKVETELQGVCDTVLGLLDSHLIKEAGDAESRVFYLKMKGDYYRYLAEVATGDDK; KRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAKTTFDEAMADLHTLSE; DSYKDSTLIMQLLRDNLTLWTADNAGEEGGEAPQEPQS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_006133
• RefSeq Size: 1336
• RefSeq ORF: 744
• Synonyms: YWHAS
• Locus ID: 2810
• UniProt ID: P31947
• Cytogenetics: 1p36.11
• Summary: This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis. [provided by RefSeq, Aug 2017]
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cell cycle, p53 signaling pathway