RTF2 (NM_016407) Human Mass Spec Standard

SKU PH301652

C20orf43 MS Standard C13 and N15-labeled recombinant protein (NP_057491)

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: [RC201652]
• Predicted MW: 33.9 kDa
• Protein Sequence:
  Protein Sequence

  >RC201652 protein sequence; Red=Cloning site Green=Tags(s); ; MGCDGGTIPKRHELVKGPKKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKS; AEKALGKAASHIKSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLRCC; GCVFSERALKEIKAEVCHTCGAAFQEDDVIVLNGTKEDVDVLKTRMEERRLRAKLEKKTKKPKAAESVSK; PDVSEEAPGPSKVKTGKPEEASLDSREKKTNLAPKSTAMNESSSGKAGKPPCGATKRSIADSEESEAYKS; LFTTHSSAKRSKEESAHWVTHTSYCF; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_057491
• RefSeq Size: 1672
• RefSeq ORF: 918
• Synonyms: C20orf43; CDAO5; HSPC164; RTFDC1; SHUJUN-3
• Locus ID: 51507
• UniProt ID: Q9BY42
• Cytogenetics: 20q13.31
• Summary: Replication termination factor which is a component of the elongating replisome (Probable). Required for ATR pathway signaling upon DNA damage and has a positive activity during DNA replication. Might function to facilitate fork pausing at replication fork barriers like the rDNA. May be globally required to stimulate ATR signaling after the fork stalls or encounters a lesion (Probable). Interacts with nascent DNA (PubMed:29290612).[UniProtKB/Swiss-Prot Function]