GLI1 (NM_005269) Human Mass Spec Standard

SKU PH301110

GLI1 MS Standard C13 and N15-labeled recombinant protein (NP_005260)

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: [RC201110]
• Predicted MW: 117.9 kDa
• Protein Sequence:
  Protein Sequence

  RC201110
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_005260
• RefSeq Size: 3618
• RefSeq ORF: 3318
• Synonyms: GLI; PAPA8; PPD1
• Locus ID: 2735
• UniProt ID: P08151
• Cytogenetics: 12q13.3
• Summary: This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer