NSE (ENO2) (NM_001975) Human Mass Spec Standard

CAT#: PH301085

ENO2 MS Standard C13 and N15-labeled recombinant protein (NP_001966)

Product images

Coomassie blue staining of purified ENO2 protein (Cat# TP301085). The protein was produced from HEK293T cells transfected with ENO2 cDNA clone (Cat# RC201085) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Description: ENO2 MS Standard C13 and N15-labeled recombinant protein (NP_001966)
• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC201085
• Predicted MW: 47.3 kDa
• Protein Sequence:
  Sequence Link

  >RC201085 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSIEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGVLKAVDHIN
  STIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAGAAERELPLYRHIAQLAGN
  SDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAMRLGAEVYHTLKGVIKDKYGKDATNVGDE
  GGFAPNILENSEALELVKEAIDKAGYTEKIVIGMDVAASEFYRDGKYDLDFKSPTDPSRYITGDQLGALY
  QDFVRDYPVVSIEDPFDQDDWAAWSKFTANVGIQIVGDDLTVTNPKRIERAVEEKACNCLLLKVNQIGSV
  TEAIQACKLAQENGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDE
  ARFAGHNFRNPSVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001966
• RefSeq Size: 2423
• RefSeq ORF: 1302
• Synonyms: HEL-S-279; NSE
• Locus ID: 2026
• Cytogenetics: 12p13.31
• Summary: This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation