2 Hydroxy phytanoyl CoA lyase (HACL1) (NM_001284415) Human Untagged Clone

SKU SC336581

HACL1 (untagged) - Human 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: 2 Hydroxy phytanoyl CoA lyase
• Synonyms: 2-HPCL; HPCL; HPCL2; PHYH2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336581 representing NM_001284415.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGCCGGACAGTAACTTCGCAGAGCGCAGCGAGGAGCAGGTGTCTGGTGCTAAAGTCATCGCTCAGGCC; CTGAAAACGCAAGATGTGGAGTACATATTTGGCATCGTAGGCATCCCAGTGACCGAAATCGCCATTGCT; GCCCAGCAGCTAGGCATCAAGTACATCGGGATGAGGAATGAGCAAGCGGCTTGTTATGCTGCCTCCGCG; ATTGGATATCTGACAAGCAGGCCAGGAGTCTGCCTTGTTGTTTCTGGCCCAGGTCTCATCCATGCCTTG; GGCGGTATGGCAAATGCAAACATGAACTGCTGGCCCTTGCTTGTGATTGGTGGTTCCTCTGAAAGAAAC; CAAGAAACAATGGGAGCTTTCCAGGAGTTTCCTCAGGCAGTGAGAAGCAGTATCTATGGTCGTCCAGGT; GCTTGCTATGTTGACATACCAGCAGATTTTGTGAACCTTCAGGTGAATGTGAATTCTATAAAGTACATG; GAACGCTGCATGTCACCTCCTATTAGCATGGCAGAAACCTCTGCTGTGTGCACGGCGGCTTCTGTTATT; AGGAATGCCAAACAACCCCTTCTTATCATCGGGAAAGGTGCTGCTTACGCTCATGCAGAAGAGAGTATC; AAGAAATTGGTGGAGCAATATAAACTGCCATTTTTGCCCACCCCTATGGGGAAGGGTGTTGTCCCTGAC; AACCATCCATACTGTGTAGGTGCAGCCAGATCCAGGGCTTTGCAATTTGCTGATGTAATTGTGTTATTT; GGTGCCAGACTAAATTGGATTTTACATTTTGGACTGCCTCCAAGATATCAGCCAGATGTGAAGTTTATC; CAGGTTGATATCTGTGCAGAAGAATTGGGGAATAATGTAAAGCCCGCTGTTACTTTGCTAGGAAACATA; CATGCTGTCACTAAGCAGGAACTAGCTTCTAAAAAATCCCTGCCTATGAATTATTACACAGTATTCTAC; CATGTTCAAGAACAACTACCTAGAGACTGTTTCGTGGTAAGTGAAGGAGCAAATACTATGGACATTGGA; CGGACTGTGCTTCAGAACTACCTTCCTCGTCACAGGCTTGATGCTGGTACTTTCGGAACAATGGGAGTT; GGTTTGGGATTTGCTATTGCAGCTGCCGTGGTGGCTAAAGATAGAAGCCCTGGGCAATGGATCATCTGT; GTGGAAGGAGACAGTGCATTTGGGTTTTCTGGCATGGAGGTAGAAACCATCTGCAGGTACAACTTGCCA; ATCATACTGTTGGTAGTGAATAACAATGGAATTTACCAAGGTTTTGATACAGATACTTGGAAAGAAATG; TTAAAATTTCAAGATGCTACTGCAGTGGTCCCTCCAATGTGTTTGCTGCCAAATTCACATTATGAGCAA; GTCATGACTGCATTTGGAGGCAAAGGGTATTTTGTACAAACACCAGAAGAACTCCAAAAATCCCTGAGG; CAGAGCCTAGCAGACACAACTAAACCTTCTCTTATCAACATCATGATTGAGCCACAAGCCACACGGAAG; GCCCAGGATTTTCATTGGCTGACCCGCTCTAATATGTAA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001284415
• Insert Size: 1557 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001284415.1
• RefSeq Size: 2107 bp
• RefSeq ORF: 1557 bp
• Locus ID: 26061
• UniProt ID: Q9UJ83
• Cytogenetics: 3p25.1
• MW: 56.7 kDa
• Summary: Catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (3) lacks two alternate in-frame exons in the central coding region, compared to variant 1, resulting in an isoform (c) that is shorter than isoform a.