BORA (NM_001286747) Human Untagged Clone

SKU SC336414

BORA (untagged) - Human bora, aurora kinase A activator (BORA), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: BORA
• Synonyms: C13orf34
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336414 representing NM_001286747.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGAACAAACTCTCGCCAGTCCTTCTGTTTTTAAATCAACAAAATTACCAAATAGATAAAGATGTGGAA; GACAAAAGACAAAAAGCCATTGAAGAGTTTTTCACTAAAGATGTCATCGTACCCTCTCCTTGGACTGAT; CATGAAGGGAAACAGCTTTCACAATGTCATTCCAGTAAATGCACTAACATAAATAGTGACTCTCCAGTT; GGAAAAAAGCTGACCATTCATTCTGAGAAAAGCGATGCTGCTTGTCAGACATTGCTGTCTCTTCCTGTG; GATTTTAATTTAGAAAATATATTAGGTGACTATTTTAGAGCTGATGAATTTGCAGATCAATCTCCTGGA; AACCTCAGTTCTTCATCCCTCAGAAGAAAGCTGTTTTTAGATGGGAACGGAAGCATCTCCGACTCCTTA; CCTTCGGCTTCTCCCGGAAGTCCTCACAGTGGTGTTCAAACATCACTAGAGATGTTTTATTCAATAGAT; TTGTCTCCTGTAAAGTGTAGGAGCCCCTTGCAGACACCAAGTTCGGGGCAGTTTTCTTCTAGCCCTATT; CAGGCTAGTGCAAAAAAATACAGCTTGGGAAGCATAACTAGTCCTTCGCCTATTTCTTCACCCACTTTC; TCACCAATTGAATTTCAGATAGGAGAGACTCCACTCTCAGAACAAAGGAAGTTTACTGTTCATTCTCCT; GATGCTTCATCTGGAACAAATTCTAATGGGATAACTAATCCGTGTATCAGAAGTCCTTATATAGATGGC; TGCTCGCCAATTAAAAATTGGTCTCCTATGAGACTTCAGATGTATAGTGGTGGTACTCAGTATCGGACC; TCAGTGATTCAGATACCTTTTACTCTTGAGACTCAAGGTGAAGATGAGGAAGATAAAGAGAATATTCCT; TCCACAGATGTCTCATCACCCGCCATGGATGCTGCTGGAATACACCTACGGCAGTTTAGTAATGAGGCT; TCTACCCATGGTACACATTTGGTTGTGACTGCCATGTCTGTTACACAAAATCAGTCCAGTGCTTCTGAG; AAAGAATTAGCACTGTTGCAGGATGTTGAAAGGGAGAAAGACAATAACACTGTGGATATGGTTGATCCT; ATAGAGATAGCAGATGAGACCACTTGGATTAAGGAGCCGGTTGATAATGGCAGTTTACCCATGACTGAT; TTTGTAAGTGGCATTGCCTTCAGTATTGAAAACTCTCATATGTGCATGTCACCTCTTGCTGAAAGCAGT; GTCATTCCTTGTGAAAGCAGTAACATTCAGATGGATAGTGGCTATAATACGCAGAATTGTGGAAGCAAT; ATTATGGATACAGTTGGGGCAGAAAGTTACTGCAAAGAAAGTGATGCACAAACATGTGAAGTTGAGAGT; AAATCTCAAGCATTTAATATGAAGCAAGACCACACAACACAGAGGTGTTGGATGAAAACAGCAAGCCCT; TTTCAATGCAGCAGTCCATAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286747
• Insert Size: 1470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001286747.1
• RefSeq Size: 2847 bp
• RefSeq ORF: 1470 bp
• Locus ID: 79866
• UniProt ID: Q6PGQ7
• Cytogenetics: 13q21.33
• Protein Families: Druggable Genome
• MW: 53.4 kDa
• Summary: BORA is an activator of the protein kinase Aurora A (AURKA; MIM 603072), which is required for centrosome maturation, spindle assembly, and asymmetric protein localization during mitosis (Hutterer et al., 2006 [PubMed 16890155]).[supplied by OMIM, Mar 2008]; Transcript Variant: This variant (3) lacks an alternate exon in the 5' coding region and initiates translation at an alternate downstream start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1.