C19orf28 (MFSD12) (NM_001287529) Human Untagged Clone

SKU SC336316

MFSD12 (untagged) - Human major facilitator superfamily domain containing 12 (MFSD12), transcript variant 4

Product Data

• Type: Human Untagged Clone
• Target Symbol: C19orf28
• Synonyms: C19orf28; PP3501
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336316 representing NM_001287529.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGGCCCGGGACCCCCAGCGGCCGGAGCGGCGCCGTCCCCGCGGCCGCTGTCCCTGGTGGCGCGGCTG; AGCTACGCCGTGGGCCACTTCCTCAACGACCTGTGCGCGTCCATGTGGTTCACCTACCTGCTGCTCTAC; CTGCACTCGGTGCGCGCCTACAGCTCCCGCGGCGCGGGGCTGCTGCTGCTGCTGGGCCAGGTGGCCGAC; GGGCTGTGCACACCGCTCGTGGGCTACGAGGCCGACCGCGCCGCCAGCTGCTGCGCCCGCTACGGCACC; GTCTGCGTCCTGCTGTCCTTCCCCTTCATCTTCAGCCCCTGCCTGGGCTGTGGGGCGGCCACGCCCGAG; TGGGCTGCCCTCCTCTACTACGGCCCGTTCATCGTGATCTTCCAGTTTGGCTGGGCCTCCACACAGATC; TCCCACCTCAGCCTCATCCCGGAGCTCGTCACCAACGACCATGAGAAGGTGGAGCTCACGGCACTCAGG; TATGCGTTCACCGTGGTGGCCAACATCACCGTCTACGGCGCCGCCTGGCTCCTGCTGCACCTGCAGGGC; TCGTCGCGGGTGGAGCCCACCCAAGACATCAGCATCAGCGACCAGCTGGGGGGCCAGGACGTGCCCGTG; TTCCGGAACCTGTCCCTGCTGGTGGTGGGTGTCGGCGCCGTGTTCTCACTGCTATTCCACCTGGGCACC; CGGGAGAGGCGCCGGCCGCATGCGGAGGAGCCAGGCGAGCACACCCCCCTGTTGGCCCCTGCCACGGCC; CAGCCCCTGCTGCTCTGGAAGCACTGGCTCCGGGAGCCGGCTTTCTACCAGGTGGGCATACTGTACATG; ACCACCAGGCTCATCGTGAACCTGTCCCAGACCTACATGGCCATGTACCTCACCTACTCGCTCCACCTG; CCCAAGAAGTTCATCGCGACCATTCCCCTGGTGATGTACCTCAGCGGCTTCTTGTCCTCCTTCCTCATG; AAGCCCATCAACAAGTGCATTGGGAGGAACATGACCTACTTCTCAGGCCTCCTGGTGATCCTGGCCTTT; GCCGCCTGGGTGGCGCTGGCGGAGGGACTGGGTGTGGCCGTGTACGCAGCGGCTGTGCTGCTGGGTGCT; GGCTGTGCCACCATCCTCGTCACCTCGCTGGCCATGACGGCCGACCTCATCGGTCCCCACACGAACAGC; GGAGCGTTCGTGTACGGCTCCATGAGCTTCTTGGATAAGGTGGCCAATGGGCTGGCAGTCATGGCCATC; CAGAGCCTGCACCCTTGCCCCTCAGAGCTCTGCTGCAGGGCCTGCGTGAGCTTTTACCACTGGGCGATG; GTGGCTGTGACGGGCGGCGTGGGCGTGGCCGCTGCCCTGTGTCTCTGTAGCCTCCTGCTGTGGCCGACC; CGCCTGCGACGCTGGGACCGTGATGCCCGGCCCTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001287529
• Insert Size: 1416 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001287529.1
• RefSeq Size: 2122 bp
• RefSeq ORF: 1416 bp
• Locus ID: 126321
• UniProt ID: Q6NUT3
• Cytogenetics: 19p13.3
• Protein Families: Transmembrane
• MW: 51 kDa
• Summary: Transporter that mediates the import of cysteine into melanosomes, thereby regulating skin pigmentation (PubMed:33208952). In melanosomes, cysteine import is required both for normal levels of cystine, the oxidized dimer of cysteine, and provide cysteine for the production of the cysteinyldopas used in pheomelanin synthesis, thereby regulating skin pigmentation (PubMed:33208952). Also catalyzes import of cysteine into lysosomes in non-pigmented cells (PubMed:33208952).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (4) uses an alternate in-frame splice junction at the 3' end of the first exon compared to variant 3. The resulting isoform (d) has the same N- and C-termini but is shorter compared to isoform c.