GRB 14 (GRB14) (NM_001303422) Human Untagged Clone

SKU SC336208

GRB14 (untagged) - Human growth factor receptor-bound protein 14 (GRB14), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: GRB 14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336208 representing NM_001303422.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGAGTTTGAGTGCAAGAAGAGTCACTCTGCCTGCAATAACGCCAATAATTCTACAGAAAAGGGTGATT; AAAGTATACAGTGAAGATGAAACCAGCAGGGCTTTAGATGTACCCAGTGACATAACGGCTCGAGATGTT; TGTCAGCTGTTGATCCTGAAGAATCATTACATTGATGACCACAGCTGGACCCTTTTTGAGCACCTGCCT; CACATAGGTGTAGAAAGAACAATAGAAGACCACGAACTGGTGATTGAAGTGCTATCCAACTGGGGGATA; GAAGAAGAAAACAAACTATACTTTAGAAAAAATTATGCCAAATATGAGTTCTTTAAAAACCCAATGTAT; TTTTTTCCAGAGCATATGGTATCTTTTGCAACTGAAACCAATGGTGAAATATCCCCCACACAGATTTTG; CAGATGTTTCTGAGTTCAAGCACATATCCTGAAATTCATGGTTTCTTACATGCGAAAGAACAGGGAAAG; AAGTCTTGGAAAAAAATTTACTTTTTTCTAAGAAGATCTGGTTTATATTTTTCTACTAAAGGAACATCA; AAGGAACCGCGGCATTTGCAGTTTTTCAGCGAATTTGGCAATAGTGATATTTATGTGTCACTGGCAGGC; AAAAAAAAACATGGAGCACCGACTAACTATGGATTCTGCTTTAAGCCTAACAAAGCGGGAGGGCCCCGA; GACCTGAAAATGCTCTGTGCAGAAGAAGAGCAGAGTAGGACGTGCTGGGTGACCGCGATTAGATTGCTT; AAGTATGGCATGCAGCTGTACCAGAATTATATGCATCCATATCAAGGTAGAAGTGGCTGCAGTTCACAG; AGCATATCACCTATGAGAAGTATATCAGAGAATTCCCTGGTAGCAATGGACTTCTCAGGCCAGAAAAGC; AGAGTTATAGAAAATCCCACTGAAGCCCTTTCAGTTGCGGTTGAAGAAGGACTCGCTTGGAGGAAAAAA; GGATGTTTACGCCTGGGCACTCACGGTAGCCCCACTGCCTCTTCACAGAGCTCTGCCACAAACATGGCT; ATCCACCGGTCCCAGCCATGGTTTCACCACAAAATTTCTAGAGATGAGGCTCAGCGATTGATTATTCAG; CAAGGACTTGTGGATGGAGTTTTCTTGGTACGGGATAGTCAGAGTAACCCCAAAACTTTCGTACTGTCA; ATGAGTCATGGACAAAAAATAAAGCACTTTCAAATTATACCAGTAGAAGATGACGGTGAAATGTTCCAC; ACACTGGATGATGGCCACACAAGATTTACAGATCTAATACAGCTGGTGGAGTTCTATCAACTCAATAAG; GGCGTTCTTCCTTGCAAGTTGAAACATTATTGTGCTAGGATTGCTCTCTAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001303422
• Insert Size: 1362 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001303422.1
• RefSeq Size: 2165 bp
• RefSeq ORF: 1362 bp
• Locus ID: 2888
• UniProt ID: Q14449
• Cytogenetics: 2q24.3
• MW: 51.9 kDa
• Summary: The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene may play a role in signaling pathways that regulate growth and metabolism. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]; Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.