D2HGDH (NM_001287249) Human Untagged Clone

SKU SC335794

D2HGDH (untagged) - Human D-2-hydroxyglutarate dehydrogenase (D2HGDH), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: D2HGDH
• Synonyms: D2HGD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335794 representing NM_001287249.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGTGGGTGGCAGCGTCCCCGTCTTTGACGAGATCATCCTCTCCACTGCCCGCATGAACCGGGTCCTC; AGCTTCCACAGCGTGTCTGGAATTCTGGTTTGCCAGGCGGGCTGCGTCCTGGAGGAGCTGAGCCGGTAT; GTGGAGGAACGGGACTTCATCATGCCGCTGGACTTAGGAGCCAAGGGCAGCTGCCACATCGGGGGAAAC; GTGGCAACCAACGCTGGAGGCCTGCGGTTTCTTCGATATGGCTCACTGCATGGGACTGTCCTGGGCCTG; GAAGTGGTGCTGGCCGACGGCACTGTCCTGGACTGCCTGACCTCCCTGAGGAAGGACAACACGGGCTAT; GACCTGAAGCAGCTGTTCATCGGGTCGGAGGGCACTTTGGGGATCATCACCACGGTGTCCATCTTGTGT; CCACCCAAGCCCAGGGCTGTGAACGTGGCTTTCCTCGGCTGCCCAGGCTTTGCTGAGGTTCTGCAGACC; TTCAGCACCTGCAAGGGGATGCTGGGTGAGATCCTGTCTGCATTCGAGTTCATGGATGCTGTGTGCATG; CAGCTGGTCGGGCGCCATCTCCACCTGGCCAGCCCGGTGCAAGAGAGTCCGTTTTACGTCCTCATCGAG; ACTTCAGGCTCCAACGCAGGCCATGACGCTGAGAAGCTGGGCCACTTCCTGGAGCACGCGCTGGGCTCC; GGCCTGGTGACCGATGGGACCATGGCCACCGACCAGAGGAAAGTCAAGATGCTGTGGGCCCTGAGGGAA; AGGATCACAGAGGCGCTGAGCCGGGATGGCTACGTGTACAAGTACGACCTCTCCCTCCCTGTGGAGCGG; CTCTACGACATCGTGACTGACCTGCGCGCCCGCCTCGGCCCGCACGCCAAGCACGTGGTGGGCTATGGC; CACCTTGGAGATGGTAACCTGCACCTCAATGTGACGGCGGAGGCCTTCAGCCCCTCGCTCCTGGCTGCC; CTGGAGCCCCACGTGTACGAGTGGACGGCCGGGCAGCAGGGCAGCGTCAGCGCGGAGCACGGAGTGGGC; TTCAGGAAGAGGGACGTCCTGGGCTACAGCAAGCCACCGGGGGCCCTGCAGCTCATGCAGCAGCTCAAG; GCCCTGCTGGACCCCAAGGGCATCCTCAACCCCTACAAGACGCTGCCCAGCCAGGCCTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001287249
• Insert Size: 1164 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001287249.1
• RefSeq Size: 2276 bp
• RefSeq ORF: 1164 bp
• Locus ID: 728294
• UniProt ID: Q8N465
• Cytogenetics: 2q37.3
• MW: 41.8 kDa
• Summary: This gene encodes D-2hydroxyglutarate dehydrogenase, a mitochondrial enzyme belonging to the FAD-binding oxidoreductase/transferase type 4 family. This enzyme, which is most active in liver and kidney but also active in heart and brain, converts D-2-hydroxyglutarate to 2-ketoglutarate. Mutations in this gene are present in D-2-hydroxyglutaric aciduria, a rare recessive neurometabolic disorder causing developmental delay, epilepsy, hypotonia, and dysmorphic features. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.