COX18 (NM_001300729) Human Untagged Clone

SKU SC335433

COX18 (untagged) - Human COX18 cytochrome c oxidase assembly factor (COX18), transcript variant 4

Product Data

• Type: Human Untagged Clone
• Target Symbol: COX18
• Synonyms: COX18HS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335433 representing NM_001300729.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGCGCTTTATGCCCGCTGTTGCTGGCGCGTGCGCGTATCAGCCTTGGCTGGCCGGTGTATGTCCGCTG; GATTGTGAGGCCCGGGATCTGGGCTCCGCCGTGGTGCAGAAATGCTGTGCCGGCTCGGCGGTCGGTGGC; TGCGGCCGCTCCCTGCCCTGCAGCTTTGGGCTAGGGACCTGCCGCTTGCGCCGGTTCCTACGAGCGGCG; CCAAGCGCCCCACTCTCCCAGTGTGGGCAGTGGCACCAGTCTCTGCAGTACATGCGAACGGCTGGTACG; AGGCCCTGGCCGCGTCTTCGCCGGTGCGGGTTGCGGAGGAAGTACTGCTCGGCGTGCACGCCGCCACGG; GCCTGCCCTGGTGGGGCAGCATTCTGCTCTCCACCGTGGCCTTACGGGGTGCTGTCACGCTGCCTTTGG; CAGCCTACCAGCACTACATCCTGGCCAAGGCTCACTTATCTAAAGAATATGAGGAGGCTAATTTCAGAG; CTATATGTGCGAGATAACTGCCACCCTTTCAAAGCCACTGTGTTGGTTTGGATTCAGCTTCCAATGTGG; ATCTTCATGTCTTTTGCTCTCCGGAATTTAAGCACGGGGGCAGCACATTCAGAAGGTTTTTCTGTTCAG; GAACAGTTAGCTACTGGTGGAATTCTGTGGTTTCCTGACCTCACTGCACCCGACTCCACTTGGATTCTG; CCTATCTCTGTTGGCGTCATCAATTTGTTAATAGTGGAGATTTGTGCTCTACAAAAAATTGGAATGTCT; CGTTTTCAGACGTATATTACGTACTTTGTCCGTGCAATGTCGGTGTTGATGATACCAATTGCTGCAACG; GTACCCTCATCAATTGTTCTCTACTGGTTATGCTCCAGCTTCGTGGGCCTTTCACAGAATTTGCTGCTG; CGTTCTCCTGGATTTCGCCAACTTTGCCGAATACCATCGACCAAGTCAGATTCAGAAACTCCTTATAAA; GACATATTTGCTGCCTTTAATACCAAGTTCATTTCAAGAAAATGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001300729
• Insert Size: 1011 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001300729.1
• RefSeq Size: 4429 bp
• RefSeq ORF: 1011 bp
• Locus ID: 285521
• UniProt ID: Q8N8Q8
• Cytogenetics: 4q13.3
• Protein Families: Transmembrane
• MW: 37.3 kDa
• Summary: This gene encodes a cytochrome c oxidase assembly protein. The encoded protein is essential for integral membrane protein insertion into the mitochondrial inner membrane. It is also required for cytochrome c oxidase assembly and activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]; Transcript Variant: This variant (4) lacks an exon in the central coding region, which results in the use of an alternate start codon and a frameshift, compared to variant 1. The encoded isoform (4) has a longer and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.