GFPT1 (NM_002056) Human Untagged Clone

SKU SC333281

GFPT1 (untagged) - Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: GFPT1
• Synonyms: CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1; GFAT1m; GFPT; GFPT1L; MSLG
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC333281 representing NM_002056.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; ATGTGTGGTATATTTGCTTACTTAAACTACCATGTTCCTCGAACGAGACGAGAAATCCTGGAGACCCTA; ATCAAAGGCCTTCAGAGACTGGAGTACAGAGGATATGATTCTGCTGGTGTGGGATTTGATGGAGGCAAT; GATAAAGATTGGGAAGCCAATGCCTGCAAAATCCAGCTTATTAAGAAGAAAGGAAAAGTTAAGGCACTG; GATGAAGAAGTTCACAAGCAACAAGATATGGATTTGGATATAGAATTTGATGTACACCTTGGAATAGCT; CATACCCGTTGGGCAACACATGGAGAACCCAGTCCTGTCAATAGCCACCCCCAGCGCTCTGATAAAAAT; AATGAATTTATCGTTATTCACAATGGAATCATCACCAACTACAAAGACTTGAAAAAGTTTTTGGAAAGC; AAAGGCTATGACTTCGAATCTGAAACAGACACAGAGACAATTGCCAAGCTCGTTAAGTATATGTATGAC; AATCGGGAAAGTCAAGATACCAGCTTTACTACCTTGGTGGAGAGAGTTATCCAACAATTGGAAGGTGCT; TTTGCACTTGTGTTTAAAAGTGTTCATTTTCCCGGGCAAGCAGTTGGCACAAGGCGAGGTAGCCCTCTG; TTGATTGGTGTACGGAGTGAACATAAACTTTCTACTGATCACATTCCTATACTCTACAGAACAGGCAAA; GACAAGAAAGGAAGCTGCAATCTCTCTCGTGTGGACAGCACAACCTGCCTTTTCCCGGTGGAAGAAAAA; GCAGTGGAGTATTACTTTGCTTCTGATGCAAGTGCTGTCATAGAACACACCAATCGCGTCATCTTTCTG; GAAGATGATGATGTTGCAGCAGTAGTGGATGGACGTCTTTCTATCCATCGAATTAAACGAACTGCAGGA; GATCACCCCGGACGAGCTGTGCAAACACTCCAGATGGAACTCCAGCAGATCATGAAGGGCAACTTCAGT; TCATTTATGCAGAAGGAAATATTTGAGCAGCCAGAGTCTGTCGTGAACACAATGAGAGGAAGAGTCAAC; TTTGATGACTATACTGTGAATTTGGGTGGTTTGAAGGATCACATAAAGGAGATCCAGAGATGCCGGCGT; TTGATTCTTATTGCTTGTGGAACAAGTTACCATGCTGGTGTAGCAACACGTCAAGTTCTTGAGGAGCTG; ACTGAGTTGCCTGTGATGGTGGAACTAGCAAGTGACTTCCTGGACAGAAACACACCAGTCTTTCGAGAT; GATGTTTGCTTTTTCCTTAGTCAATCAGGTGAGACAGCAGATACTTTGATGGGTCTTCGTTACTGTAAG; GAGAGAGGAGCTTTAACTGTGGGGATCACAAACACAGTTGGCAGTTCCATATCACGGGAGACAGATTGT; GGAGTTCATATTAATGCTGGTCCTGAGATTGGTGTGGCCAGTACAAAGGCTTATACCAGCCAGTTTGTA; TCCCTTGTGATGTTTGCCCTTATGATGTGTGATGATCGGATCTCCATGCAAGAAAGACGCAAAGAGATC; ATGCTTGGATTGAAACGGCTGCCTGATTTGATTAAGGAAGTACTGAGCATGGATGACGAAATTCAGAAA; CTAGCAACAGAACTTTATCATCAGAAGTCAGTTCTGATAATGGGACGAGGCTATCATTATGCTACTTGT; CTTGAAGGGGCACTGAAAATCAAAGAAATTACTTATATGCACTCTGAAGGCATCCTTGCTGGTGAATTG; AAACATGGCCCTCTGGCTTTGGTGGATAAATTGATGCCTGTGATCATGATCATCATGAGAGATCACACT; TATGCCAAGTGTCAGAATGCTCTTCAGCAAGTGGTTGCTCGGCAGGGGCGGCCTGTGGTAATTTGTGAT; AAGGAGGATACTGAGACCATTAAGAACACAAAAAGAACGATCAAGGTGCCCCACTCAGTGGACTGCTTG; CAGGGCATTCTCAGCGTGATCCCTTTACAGTTGCTGGCTTTCCACCTTGCTGTGCTGAGAGGCTATGAT; GTTGATTTCCCACGGAATCTTGCCAAATCTGTGACTGTAGAGTGA;
• Restriction Sites: SgfI-MluI
• ACCN: NM_002056
• Insert Size: 2046 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002056.3
• RefSeq Size: 8649 bp
• RefSeq ORF: 2046 bp
• Locus ID: 2673
• UniProt ID: Q06210
• Cytogenetics: 2p13.3
• Domains: GATase_2, SIS
• Protein Families: Protease
• Protein Pathways: Alanine, Amino sugar and nucleotide sugar metabolism, aspartate and glutamate metabolism, Metabolic pathways
• MW: 76.8 kDa
• Summary: This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate. [provided by RefSeq, Sep 2008]; Transcript Variant: This variant (2) lacks an alternate exon in the coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.