AP3D1 (NM_001261826) Human Untagged Clone

SKU SC332796

AP3D1 (untagged) - Homo sapiens adaptor-related protein complex 3, delta 1 subunit (AP3D1), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: AP3D1
• Synonyms: ADTD; hBLVR; HPS10
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332796 representing NM_001261826.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; ATGGCCCTCAAGATGGTGAAGGGCAGCATCGACCGCATGTTCGACAAGAATCTGCAGGACTTGGTCCGC; GGCATCCGTAACCACAAGGAGGACGAGGCAAAATACATATCTCAGTGCATTGATGAGATCAAGCAGGAG; CTGAAGCAGGACAACATAGCGGTGAAGGCGAACGCGGTCTGCAAGCTGACGTATTTACAGATGTTGGGA; TACGACATCAGCTGGGCCGCCTTCAACATCATAGAAGTGATGAGTGCCTCCAAGTTCACCTTCAAGCGA; ATTGGCTACCTCGCTGCTTCCCAGAGCTTTCACGAAGGCACCGACGTCATCATGCTGACCACCAATCAG; ATCCGTAAGGACTTGAGCAGCCCCAGCCAGTACGACACAGGTGTTGCACTGACGGGTCTGTCCTGCTTC; GTCACCCCAGACCTTGCCAGAGACCTGGCAAATGACATCATGACACTGATGTCACACACCAAGCCCTAC; ATCAGGAAGAAGGCTGTGCTGATCATGTACAAGGTGTTCCTGAAGTACCCCGAGTCGCTGCGCCCTGCC; TTTCCCCGGCTGAAGGAGAAGCTGGAGGACCCCGACCCCGGGGTTCAGTCGGCTGCCGTCAATGTCATC; TGCGAGCTGGCCAGACGCAACCCTAAGAACTACCTGTCCCTGGCCCCGCTCTTTTTCAAGCTGATGACG; TCCTCCACCAACAACTGGGTCCTCATCAAGATCATCAAGCTGTTCGGTGCTCTTACTCCTTTGGAACCG; CGGCTGGGCAAGAAGCTGATCGAGCCCCTCACCAATCTCATCCACAGCACGTCTGCCATGTCTCTCCTC; TATGAATGTGTGAACACCGTGATTGCAGTGCTCATCTCGCTGTCCTCCGGCATGCCCAACCACAGCGCC; AGCATCCAGCTTTGTGTTCAGAAATTAAGGATATTGATCGAGGACTCCGATCAGAACTTGAAGTACCTG; GGGCTGCTGGCAATGTCCAAGATCCTGAAGACCCACCCCAAGTCCGTGCAGTCCCACAAGGACCTCATC; CTGCAGTGCCTGGACGACAAGGACGAGTCCATCCGGCTGCGGGCCCTGGACCTGCTCTATGGGATGGTG; TCCAAGAAGAACCTGATGGAGATCGTGAAGAAGCTGATGACCCACGTAGACAAGGCAGAGGGTACCACC; TACCGTGACGAGCTGCTCACCAAGATCATTGACATCTGCAGCCAGTCCAACTACCAGTACATCACCAAC; TTCGAGTGGTACATCAGCATCCTGGTGGAGCTGACCCGGCTGGAGGGCACACGGCACGGCCACCTCATC; GCCGCCCAAATGCTGGACGTGGCCATCCGCGTGAAGGCCATCCGCAAGTTCGCCGTGTCCCAGATGTCT; GCGCTGCTTGACAGTGCACACCTGCTGGCCAGCAGCACCCAGCGGAACGGGATCTGTGAGGTGCTGTAC; GCTGCCGCCTGGATCTGCGGGGAGTTCTCAGAGCATCTGCAGGAACCACACCACACTTTGGAGGCCATG; CTGCGGCCCAGAGTCACCACGCTGCCAGGCCACATCCAGGCCGTGTATGTGCAGAACGTGGTCAAGCTC; TACGCCTCCATCCTGCAGCAGAAGGAGCAGGCCGGGGAGGCAGAGGGCGCTCAGGCCGTCACCCAGCTC; ATGGTGGACCGGCTGCCCCAGTTTGTGCAGAGCGCAGACCTGGAGGTGCAGGAGCGGGCGTCCTGCATC; CTGCAGCTGGTCAAGCACATCCAGAAGCTTCAGGCCAAGGACGTGCCTGTGGCAGAGGAGGTCAGCGCT; CTCTTTGCTGGGGAGCTGAACCCAGTGGCCCCCAAGGCCCAGAAGAAGGTTCCAGTCCCCGAAGGCCTG; GACCTGGACGCCTGGATCAATGAGCCACTCTCGGACAGCGAGTCAGAGGACGAGAGGCCCAGGGCCGTC; TTCCACGAGGAGGAGCAGCGGCGTCCCAAGCACCGGCCGTCGGAGGCGGACGAGGAAGAGCTGGCTCGG; CGCCGAGAGGCCCGGAAGCAGGAGCAGGCCAACAACCCCTTCTACATCAAGAGCTCGCCATCGCCACAG; AAGCGGTACCAGGACACCCCGGGCGTGGAGCACATTCCCGTGGTGCAGATTGACCTCTCCGTCCCCTTG; AAGGTTCCAGGGCTGCCTATGTCAGATCAGTATGTGAAGCTGGAGGAGGAGCGGCGGCACCGGCAGAAG; CTGGAGAAGGACAAGAGGAGGAAAAAGAGGAAGGAGAAGGAGAAGAAGGGCAAGCGCCGCCACAGCTCG; CTGCCCACGGAGAGCGACGAGGACATCGCCCCTGCCCAGCAGGTGGACATCGTCACAGAGGAGATGCCT; GAGAATGCTCTGCCCAGCGACGAGGATGACAAAGACCCCAACGACCCCTACAGGGCTCTGGATATTGAC; CTGGATAAGCCCTTAGCCGACAGCGAGAAACTGCCTATTCAGAAACACAGAAACACCGAGACCTCAAAA; TCCCCTGAGAAGGACGTTCCCATGGTAGAAAAGAAGAGCAAGAAACCCAAGAAGAAAGAGAAAAAACAC; AAAGAGAAAGAGAGAGACAAGGAGAAGAAGAAGGAGAAGGAGAAGAAGGCTGAGGACCTGGACTTCTGG; CTGTCTACCACCCCACCGCCTGCCCCCGCCCCCGCCCCCGCCCCCGTTCCATCCACGGGGGAGCTCAGT; GTGAACACTGTCACTACCCCGAAGGACGAGTGTGAGGACGCCAAGACGGAGGCGCAGGGCGAGGAGGAC; GATGCCGAGGGGCAAGACCAGGACAAGAAATCTCCCAAGCCTAAGAAGAAGAAGCACAGGAAGGAGAAG; GAGGAGCGGACCAAAGGCAAGAAGAAGTCCAAGAAGCAGCCTCCAGGCAGCGAGGAGGCAGCGGGGGAG; CCGGTGCAGAATGGCGCGCCAGAGGAGGAGCAGCTCCCGCCTGAGTCCAGCTACTCCCTCCTCGCTGAA; AATTCCTATGTTAAAATGACCTGTGACATCCGGGGCAGTCTGCAGGAGGACAGCCAGGTCACTGTGGCC; ATCGTGCTGGAGAACAGGAGCAGCAGCATCCTCAAGGGCATGGAGCTCAGCGTGCTGGACTCACTCAAT; GCCAGGATGGCCCGGCCGCAGGGCTCCTCCGTCCACGATGGCGTCCCCGTGCCTTTCCAGCTGCCCCCA; GGCGTCTCCAACGAAGCCCAGTATGTGTTCACCATCCAGAGCATCGTCATGGCGCAGAAGCTCAAGGGG; ACCCTGTCCTTCATTGCCAAGAATGACGAGGGTGCGACCCACGAGAAGCTGGACTTCAGGCTGCACTTC; AGCTGCAGCTCCTACTTGATCACCACTCCCTGCTACAGTGACGCCTTTGCTAAGTTGCTGGAGTCTGGG; GACTTGAGCATGAGCTCAATCAAAGTCGATGGCATTCGGATGTCCTTCCAGAATCTTCTGGCGAAGATC; TGTTTTCACCACCATTTTTCCGTTGTGGAGCGAGTGGACTCCTGCGCCTCCATGTACAGCCGCTCCATC; CAGGGCCACCATGTCTGCCTCCTGGTGAAAAAGGGTGAGAACTCTGTCTCAGTCGACGGGAAGTGCAGT; GACTCCACGCTACTGAGCAACTTGTTAGAAGAGATGAAGGCGACGCTGGCCAAGTGTTGA;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001261826
• Insert Size: 3648 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001261826.1
• RefSeq Size: 5073 bp
• RefSeq ORF: 3648 bp
• Locus ID: 8943
• UniProt ID: O14617
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome
• MW: 136.7 kDa
• Summary: The protein encoded by this gene is a subunit of the AP3 adaptor-like complex, which is not clathrin-associated, but is associated with the golgi region, as well as more peripheral structures. The AP-3 complex facilitates the budding of vesicles from the golgi membrane, and may be directly involved in trafficking to lysosomes. This subunit is implicated in intracellular biogenesis and trafficking of pigment granules, and possibly platelet dense granules and neurotransmitter vesicles. Defects in this gene are a cause of a new type of Hermansky-Pudlak syndrome. [provided by RefSeq, Feb 2017]; Transcript Variant: This variant (3) encodes the longer isoform (3).